Microplate Instrumentation Specialists

          Ordering Information
          Phone   1.800.848.6474   
          Phone 01.703.821.1045
          Fax      01.703.821.1046
          Email   info@mtxlsi.com

Additional in-stock Fluorescence Plate Readers:
|Cytofluor 4000|BMG Fluostar|BMG Fluorstar Galaxy|

|Tecan Spectrafluor|Tecan Spectrafluor Plus|

|Perkin Elmer Victor 2 w/dispenser|Perkin Elmer Victor 2|

|Perkin Elmer Victor|Perkin Elmer HTS 7000|

|Thermo Electron/Labsystems Finstruments Fluorescence|

|Fluoroskan II|Fluoroskan I|Accessories|
Gemini EM Monochromatic
Multi-label Absorbance, Fluorescence
 & Luminescence Plate Reader SOLD


 
The Gemini EM spectrofluorometer allows for fast, high-sensitivity measurements. Cellular and molecular binding studies, DNA quantitation, drug screening, and SNP genotyping analysis all require a high degree of sensitivity. The Gemini system offers a flexible, filter-free environment allowing the determination of optimal excitation and emission settings in 1.0 nm increments providing the best level of sensitivity for variable assay conditions.
 
 

Applications

    Cellular and molecular binding studies
    Nucleic acid quantitation
    Cytoxicity assays
    SNP genotype detection and molecular beacons
    Enzyme activity
    CatchPoint® cAMP Assay Kit

Features

    Top and Bottom Reading Optics-The top/bottom reading optical design of the Gemini EM allows for measurements for both solution and cell-based assays.
    Dual Monochromators — The right pair of excitation and emission wavelengths is always available because the dual monochromators allow the selection of any wavelength in 1 nm increments. New fluorophores can easily be evaluated without purchasing additional filters.
    Wavelength Scanning — The most sensitive results are achieved by using optimal excitation and emission wavelengths. Literature wavelengths are often based on results from wavelength-limited, filter-based readers. Wavelength scanning ensures that the most sensitive assay conditions are used.
    Well Scanning — Gemini EM can report a single point from the well center, or multiple data points from large well tissue culture plates. This provides high sensitivity for cell-based assays.
    Auto PMT Gain — Because a single microplate often presents a range of fluorescence intensities greater than three orders of magnitude, Gemini EM features “Auto PMT Gain” to avoid saturating the photomultiplier tube. The signal is calibrated against an internal standard, so the reported RFU values of individual samples can be accurately compared.
 

 
 

SoftMax Pro software

SoftMax Pro allows you to select excitation and emission values and simply input their values for the monochromator settings. You can easily scan both excitation and emission spectra and determine the optimal settings.  Software not included.

Engineered for superior performance and reliability

Gemini EM dual monochromator design — an excitation monochromator (A) and emission monochromator (B) — allows user-defined variable wavelength settings.  Sending light through a movable scanning grating (D) which selects the desired excitation wavelength. The light is passed through 1 mm fiber optic bundles (E) to elliptical focusing mirrors (F) that maximize light collection efficiency for superior sensitivity and send the beam to a microplate well (G). If fluorescent molecules are present, the light of the emission wavelength is emitted back out to focusing mirrors (H) that direct the light through 4 mm fiber optic bundles (J) to the emission scanning grating (K). The desired wavelength of light is then detected by the photomultiplier tube, or PMT (L). The PMT passes the fluorescence signal through the Gemini’s electronics, creating the signals that result in data points collected by software.
 

 
 

Specifications**
 

Fluorescence photometric performance
Dual Scanning Monochromators 1 nm increment selection
Excitation Wavelength 250-850 nm
Emission Wavelength 360-850 nm
Bandwidth (Excitation & Emission) 9 nm
Top Read Sensitivity 3.0 fmol/well FITC 200 µl
Bottom Read Sensitivity
(Signal 3x Std. Dev. of Baseline		 8.0 fmol/well FITC 200 µl

Time Resolved Fluorescence (secondary mode)
Data Collection 50-1450 µl sec, 200 µl sec increments
Sensitivity 0.5 fmol/well

Luminescence Performance
Wavelength Range                                  250-850nm
Detection Limit 10 amol/well Alkaline Phosphatase 200 µl/well

General photometric specifications
Microplate Formats 6-, 12-, 24-, 48-, 96-, and 384-well
Light Source Xenon flash lamp (1 joule/flash)
Detector Photomultiplier (R-3896)
Read Time*** 96 wells in <15 seconds
384 wells in <45 seconds
Shaker Time 0 to 999 seconds
Temperature Control (Chamber) Ambient + 4 °C, to 45 °C
Gemini EM Catalog #P97045 Remanufactured

Comes complete with line cord, six days a week technical support
NIST documentation and certification, and one year warranty. 
Softmax Pro PC and MAC Software additional.