Additional in-stock
Multimode Detection and Fluorescence Plate Readers:
|Fluoroskan I|Perseptive Biosystems Cytofluor 4000|BMG
FLUOstar|
|Biotek
FL 600|
|ThermoFisher Finstruments Fluorescence|ThermoFisher
Ascent|Perkin Elmer|
|Tecan
Spectrafluor|Tecan
Spectrafluor Plus|Tecan
Genios|
|Molecular Devices Gemini|Molecular
Devices Gemini XS|
|BMG
FLUOstar Galaxy|Perkin
Elmer Victor 2 with or w/o injectors|
|Thermo Fisher
Ascent FL with w/o injectors|Tecan
ULTRA|
|Molecular
Devices Gemini EM|
|BMG POLARstar
Galaxy with injectors|
Molecular Devices
Spectramax Gemini EM Monochromatic
Multifunction Fluorescence, TRF, FI, and Luminescence Microplate
Reader
Equipped with UV-VIS
Fluorescence and Luminescence Reader
for top-
and bottom- read assays $17,795.
Brand new!
|
| The Gemini EM spectrofluorometer allows
for fast, high-sensitivity measurements. Cellular and molecular
binding studies, DNA quantitation, drug screening, and SNP
genotyping analysis all require a high degree of sensitivity. The
Gemini EM system offers a flexible, filter-free environment
allowing the determination of optimal excitation and emission
settings in 1.0 nm increments providing the best level of
sensitivity for variable assay conditions. Improved top and bottom optics design for greater sensitivity in
cell-based assays; fluorescence and luminescence; tunable
wavelength selection for excitiation and emission from 250 nm to
850 nm in 1 nm increments; thermal regulation from ambient +1C to
45C. |
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Applications
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Cellular
and molecular binding studies |
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Nucleic
acid quantitation |
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Cytoxicity
assays |
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SNP
genotype detection and molecular beacons |
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Enzyme
activity |
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CatchPoint® cAMP Assay Kit |
Features
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Top and Bottom Reading Optics-The
top/bottom reading optical design of the Gemini EM allows for
measurements for both solution and cell-based assays. |
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Dual
Monochromators — The right pair
of excitation and emission wavelengths is always available
because the dual monochromators allow the selection of any
wavelength in 1 nm increments. New fluorophores can easily be
evaluated without purchasing additional filters. |
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Wavelength
Scanning — The most sensitive
results are achieved by using optimal excitation and emission
wavelengths. Literature wavelengths are often based on results
from wavelength-limited, filter-based readers. Wavelength
scanning ensures that the most sensitive assay conditions are
used. |
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Well
Scanning — Gemini EM can report
a single point from the well center, or multiple data points
from large well tissue culture plates. This provides high
sensitivity for cell-based assays. |
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Auto PMT
Gain — Because a single
microplate often presents a range of fluorescence intensities
greater than three orders of magnitude, Gemini EM features
“Auto PMT Gain” to avoid saturating the photomultiplier tube.
The signal is calibrated against an internal standard, so the
reported RFU values of individual samples can be accurately
compared. |
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SoftMax Pro software
SoftMax Pro allows you to select
excitation and emission values and simply input their values for
the monochromator settings. You can easily scan both excitation
and emission spectra and determine the optimal settings.
Engineered for superior performance and reliability
Gemini EM dual monochromator design —
an excitation monochromator (A) and emission monochromator (B) —
allows user-defined variable wavelength settings. Sending light
through a movable scanning grating (D) which selects the desired
excitation wavelength. The light is passed through 1 mm fiber
optic bundles (E) to elliptical focusing mirrors (F) that maximize
light collection efficiency for superior sensitivity and send the
beam to a microplate well (G). If fluorescent molecules are
present, the light of the emission wavelength is emitted back out
to focusing mirrors (H) that direct the light through 4 mm fiber
optic bundles (J) to the emission scanning grating (K). The
desired wavelength of light is then detected by the
photomultiplier tube, or PMT (L). The PMT passes the fluorescence
signal through the Gemini’s electronics, creating the signals that
result in data points collected by software. |
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Specifications** |
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Fluorescence photometric performance |
| Dual
Scanning Monochromators |
1 nm
increment selection |
| Excitation
and Emission Wavelength |
250-850
nm |
| Bandwidth (Excitation & Emission) |
9 nm |
| Top Read
Sensitivity |
3.0 fmol/well
FITC 200 µl |
Bottom
Read Sensitivity
(Signal 3x Std. Dev. of Baseline |
8.0 fmol/well
FITC 200 µl |
Time Resolved Fluorescence
Luminescence Performance |
| Wavelength
Range 250-850nm |
| Detection Limit |
10 amol/well
Alkaline Phosphatase 200 µl/well |
| Glow
LUminescence |
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General photometric specifications |
| Microplate Formats |
6-, 12-,
24-, 48-, 96-, and 384-well |
| Light
Source |
Xenon
flash lamp (1 joule/flash) |
| Detector |
Photomultiplier (R-3896) |
| Read
Time*** |
96 wells
in <15 seconds
384 wells in <45 seconds |
| Shaker
Time |
0 to 999
seconds |
| Temperature Control (Chamber) |
Ambient
+ 4 °C, to 45 °C |
|
Catalog #6723117 |
Softmax
Pro PC/MAC Software via interface connections
RS 232 serial
& parallel port |
|
Catalog #6723119 |
SpectraTest ABS 1
validation package |
| Catalog #P97045 |
Brand new! |
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Comes complete with line
cord, six days a week technical support,
NIST documentation and certification,
and full one year warranty.
Softmax Pro PC/MAC Software additional. |
