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Additional in-stock Multi-mode Detection
and Fluorescence Plate Readers:
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|Fluoroskan I|Perseptive Biosystems Cytofluor 4000|BMG FLUOstar|

|Biotek FL 600|Dynex Technologies MLX|

|ThermoFisher Finstruments Fluorescence|ThermoFisher Ascent|

|Tecan Spectrafluor|Tecan Spectrafluor Plus|Tecan Genios|

|Molecular Devices Gemini XS|ThermoFisher Luminoskan|

|ThermoFisher Luminoskan Ascent|

|BMG FLUOstar Galaxy|Perkin Elmer Victor 2 with or w/o injectors|

|BMG POLARstar|

|BMG POLARstar Galaxy|Biotek FLX 800|BMG NOVOstar|Tecan Ultra|

|Tecan Genios Pro|Thermo Fisher Ascent FL with w/o injectors|

 |Molecular Devices M5|Molecular Devices Gemini EM|Tecan Safire|


Molecular Devices Spectramax Gemini EM Monochromatic
 Fluorescence, TRF, FI, and Luminescence Microplate Reader
 for top- and bottom- read assays

Brand new with 1 year warranty!


 

The Gemini EM spectrofluorometer allows for fast, high-sensitivity measurements. Cellular and molecular binding studies, DNA quantitation, drug screening, and SNP genotyping analysis all require a high degree of sensitivity. The Gemini EM system offers a flexible, filter-free environment allowing the determination of optimal excitation and emission settings in 1.0 nm increments providing the best level of sensitivity for variable assay conditions.  Improved top and bottom optics design for greater sensitivity in cell-based assays; fluorescence and luminescence; tunable wavelength selection for excitiation and emission from 250 nm to 850 nm in 1 nm increments; thermal regulation from ambient +1C to 45C.
 
 

Applications

    Cellular and molecular binding studies
    Nucleic acid quantitation
    Cytoxicity assays
    SNP genotype detection and molecular beacons
    Enzyme activity
    CatchPoint® cAMP Assay Kit

Features

    Top and Bottom Reading Optics-The top/bottom reading optical design of the Gemini EM allows for measurements for both solution and cell-based assays.
    Dual MonochromatorsThe right pair of excitation and emission wavelengths is always available because the dual monochromators allow the selection of any wavelength in 1 nm increments. New fluorophores can easily be evaluated without purchasing additional filters.
    Wavelength ScanningThe most sensitive results are achieved by using optimal excitation and emission wavelengths. Literature wavelengths are often based on results from wavelength-limited, filter-based readers. Wavelength scanning ensures that the most sensitive assay conditions are used.
    Well ScanningGemini EM can report a single point from the well center, or multiple data points from large well tissue culture plates. This provides high sensitivity for cell-based assays.
    Auto PMT GainBecause a single microplate often presents a range of fluorescence intensities greater than three orders of magnitude, Gemini EM features “Auto PMT Gain” to avoid saturating the photomultiplier tube. The signal is calibrated against an internal standard, so the reported RFU values of individual samples can be accurately compared.
 

 
 

SoftMax Pro software

SoftMax Pro allows you to select excitation and emission values and simply input their values for the monochromator settings. You can easily scan both excitation and emission spectra and determine the optimal settings. 

Engineered for superior performance and reliability

Gemini EM dual monochromator design — an excitation monochromator (A) and emission monochromator (B) — allows user-defined variable wavelength settings.  Sending light through a movable scanning grating (D) which selects the desired excitation wavelength. The light is passed through 1 mm fiber optic bundles (E) to elliptical focusing mirrors (F) that maximize light collection efficiency for superior sensitivity and send the beam to a microplate well (G). If fluorescent molecules are present, the light of the emission wavelength is emitted back out to focusing mirrors (H) that direct the light through 4 mm fiber optic bundles (J) to the emission scanning grating (K). The desired wavelength of light is then detected by the photomultiplier tube, or PMT (L). The PMT passes the fluorescence signal through the Gemini’s electronics, creating the signals that result in data points collected by software.

 

 
 

Specifications**

 

Fluorescence photometric performance

Dual Scanning Monochromators 1 nm increment selection
Excitation and Emission Wavelength 250-850 nm
Bandwidth (Excitation & Emission) 9 nm
Top Read Sensitivity 3.0 fmol/well FITC 200 µl
Bottom Read Sensitivity
(Signal 3x Std. Dev. of Baseline
8.0 fmol/well FITC 200 µl

Time Resolved Fluorescence

Luminescence Performance

Wavelength Range                                  250-850nm
Detection Limit 10 amol/well Alkaline Phosphatase 200 µl/well
Glow LUminescence  

 

Microplate Formats 6-, 12-, 24-, 48-, 96-, and 384-well
Light Source Xenon flash lamp (1 joule/flash)
Detector Photomultiplier (R-3896)
Read Time*** 96 wells in <15 seconds
384 wells in <45 seconds
Shaker Time 0 to 999 seconds
Temperature Control (Chamber) Ambient + 4 °C, to 45 °C
Catalog #6723117 Softmax Pro PC/MAC Software via interface connections RS 232 serial & parallel port
Catalog #6723119 SpectraTest ABS 1 validation package
Catalog #P97045 Brand new! 

Warranty information:

Instruments come complete with NIST certification and documentation, line cord and six days a week technical support. Remanufactured model with one year warranty.  High quality instrument made in USA. Easily reset to 220 Volt.

Microplate Instrumentation Sales, Service and Support since 1982.

 
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