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Luminoskan
Ascent $7,995.
The
Luminoskan Ascent, combined with powerful PC Software,
handles a wide range of research, industrial and clinical luminescense
applications
-
Plate formats
from 12 to 384 wells
-
Sensitive
-
Dynamic range
- more than 9 decades
-
Optional
dispensers - up to 3 can be added
-
Incubation
and shaking included with standard model
-
Powerful PC
Ascent Software included
Excellent Flexibility
For maximum versatility and
flexibility, Luminoskan Ascent reads a range of different plate formats,
from 12 to 384-well plates. The high-performance fiberless optics enables
measurement sensitivity of less than 1 fmol of ATP per well, and the dynamic
range of Luminoskan Ascent covers more than nine decades. For easy and
accurate addition of reagents, up to three dispensers can be fitted
on-board. These dispensers allow precise delivery of reagents over an
adjustable volume range of 5 - 1000 µl (not available with this model). For assays requiring sensitive
temperature control, this Luminoskan Ascent has an on-board incubator. Orbital shaking
with adjustable speed and diameter ensures effective mixing and speeds up
reaction times.
Powerful Ascent PC Software
Powerful Software allows
easy assay optimization, flexible data handling and convenient report
formatting. For flash luminescence reactions performed with acridinium
esters or aequorin, for example, software supports simultaneous reagent
injection and signal monitoring. The ability to add reagents and take
readings in any order allows for multiphase ATP and reporter gene assays.
Effective Robotic
Integration
This Ascent model is an
ideal instrument for high-volume testing, such as high throughput
screening. Luminoskan Ascent has been designed for complete robot
compatibility with the plate carrier allowing access for different types of
robotic arms. Luminoskan Ascent offers convenient combination with any
other robotic system due to the easy integration of Ascent Software with any
robotic and HMS/LIMS system.
Application areas of the
Luminoskan Ascent
When combined with Software,
the Luminoskan Ascent works well for all luminescent applications.
- Reporter gene assays,
such as:
- Eukaryotic luciferase
gene activity
- Prokaryotic bacterial
luciferase gene activity
- Promega
Dual-Luciferase Assay
- Gene Activities with
luminescent substrates
- Immunoassays with
luminescent substrates
- Alkaline phosphatease,
horseradish peroxidase, and others.
- Cytotoxicity and cell
proliferation assays
- MIC, EC, ATP assays
- Growth inhibition
assays
- Intracellular Ca2+ assays
- ATP assays
- DNA quantitation
- Biotin/streptavidin-linked
chemiluminescent assays
- Phagocytosis assays
- Reactive oxygen assays
- Microbiological assays
- Antibiotic sensitivity
testing
- Hygiene monitoring
assays
- Enzyme assay
- Connected assays via
ATP
- Assays with
chemiluminescent substrates
Robotic integration is
simple and effective with the Luminoskan Ascent. The plate carrier allows
convenient access for the robotic arm, and Software is easy to integrate
with robotic and HIS/LIMS systems. The execution of predefined assays can be
controlled remotely, and the software can be set up to export and import
data automatically.
Technical
Specifications:
|
Weight |
Basic unit 21kg. Optional 3
dispenser add 3.5 kg |
|
Overall dimensions |
420mm (W) x 420 mm (D) x
340 mm (H) |
|
Operating conditions |
+10ºC -- +40ºC, Relative
humidity 90% max. |
|
Warm-up time |
<15 min to rated accuracy |
|
Detector |
Photomultiplier tube (PMT) |
|
Spectral Response |
270-670 nm |
|
Measurement range
|
0.0001 - 5000 Relative
Light Units (RLU) |
|
Gain |
User changeable (300 - 1000
V) |
|
Plate types |
1-, 6-, 12-, 24-, 48-, 96-,
and 384-well plates
Maximum dimensions 90 mm x 134 mm x 25 mm. |
|
Measuring Speed |
Minimum measurement time
for 96-well plate, 15s |
|
Typical sensitivity |
<1.0 fmol STP/well with
white 96-well plate |
|
Dynamic range |
>9 decades over whole gain
setting area |
|
Shaker |
Orbital method, speed 60 -
1200 RPM, ø 1-50 mm |
|
Incubator |
From RT (25ºC) +3ºC to
+45ºC when ambient temperature is 25ºC |
|
Dispensers |
Optional 1-3 dispensers.
Dispensing volume
5-1000µl in 5µl increments, accuracy: ±3µl avg., precision: 5-15µl, <5%,
20-1000µl, <2%. Dispensing speed: 25 sec, 96-well plate, 5µl/well |
|
Catalog #P30016 |
Luminoskan Ascent
Remanufactured |
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