Microplate Instrumentation Specialists

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Luminoskan Ascent
|Luminoskan Model|Luminoskan Ascent|Dynex MLX Model |
|Dynex MLX Model w/3 dispensers|Perkin Elmer Victor|
 |Perkin Elmer Victor 2|Perkin Elmer Victor 2 with dispensers|
|Tecan Spectrofluor Plus|Tecan Genios|

Ascent FL

 

Luminoskan Ascent $7,995.

The Luminoskan Ascent, combined with powerful PC Software, handles a wide range of research, industrial and clinical luminescense applications

  • Plate formats from 12 to 384 wells

  • Sensitive

  • Dynamic range - more than 9 decades

  • Optional dispensers - up to 3 can be added

  • Incubation and shaking included with standard model

  • Powerful PC Ascent Software included

Excellent Flexibility

For maximum versatility and flexibility, Luminoskan Ascent reads a range of different plate formats, from 12 to 384-well plates.  The high-performance fiberless optics enables measurement sensitivity of less than 1 fmol of ATP per well, and the dynamic range of Luminoskan Ascent covers more than nine decades. For easy and accurate addition of reagents, up to three dispensers can be fitted on-board.  These dispensers allow precise delivery of reagents over an adjustable volume range of 5 - 1000 µl (not available with this model). For assays requiring sensitive temperature control, this Luminoskan Ascent has an on-board incubator.  Orbital shaking with adjustable speed and diameter ensures effective mixing and speeds up reaction times.

Powerful Ascent PC Software

Powerful Software allows easy assay optimization, flexible data handling and convenient report formatting. For flash luminescence reactions performed with acridinium esters or aequorin, for example, software supports simultaneous reagent injection and signal monitoring.  The ability to add reagents and take readings in any order allows for multiphase ATP and reporter gene assays.

Effective Robotic Integration

This Ascent model is an ideal instrument for high-volume testing, such as high throughput screening.  Luminoskan Ascent has been designed for complete robot compatibility with the plate carrier allowing access for different types of robotic arms. Luminoskan Ascent offers convenient combination with any other robotic system due to the easy integration of Ascent Software with any robotic and HMS/LIMS system.


Application areas of the Luminoskan Ascent

When combined with Software, the Luminoskan Ascent works well for all luminescent applications.

  • Reporter gene assays, such as:
    • Eukaryotic luciferase gene activity
    • Prokaryotic bacterial luciferase gene activity
    • Promega Dual-Luciferase Assay
    • Gene Activities with luminescent substrates
  • Immunoassays with luminescent substrates
    • Alkaline phosphatease, horseradish peroxidase, and others.
  • Cytotoxicity and cell proliferation assays
    • MIC, EC, ATP assays
    • Growth inhibition assays
  • Intracellular Ca2+ assays
    • Aequorin loading assay
  • ATP assays
    • Biomass assays
  • DNA quantitation
    • Biotin/streptavidin-linked chemiluminescent assays
  • Phagocytosis assays
  • Reactive oxygen assays
  • Microbiological assays
    • Antibiotic sensitivity testing
    • Hygiene monitoring assays
  • Enzyme assay
    • Connected assays via ATP
    • Assays with chemiluminescent substrates

Robotic integration is simple and effective with the Luminoskan Ascent. The plate carrier allows convenient access for the robotic arm, and  Software is easy to integrate with robotic and HIS/LIMS systems. The execution of predefined assays can be controlled remotely, and the software can be set up to export and import data automatically.
 
Technical Specifications:


Weight Basic unit 21kg. Optional 3 dispenser add 3.5 kg
Overall dimensions 420mm (W) x 420 mm (D) x 340 mm (H)
Operating conditions +10ºC -- +40ºC, Relative humidity 90% max.
Warm-up time <15 min to rated accuracy
Detector Photomultiplier tube (PMT)
Spectral Response 270-670 nm
Measurement range 0.0001 - 5000 Relative Light Units (RLU)
Gain User changeable (300 - 1000 V)
Plate types 1-, 6-, 12-, 24-, 48-, 96-, and 384-well plates
Maximum dimensions 90 mm x 134 mm x 25 mm.
Measuring Speed Minimum measurement time for 96-well plate, 15s
Typical sensitivity <1.0 fmol STP/well with white 96-well plate
Dynamic range >9 decades over whole gain setting area
Shaker Orbital method, speed 60 - 1200 RPM, ø 1-50 mm
Incubator From RT (25ºC) +3ºC to +45ºC when ambient temperature is 25ºC
Dispensers Optional 1-3 dispensers.  Dispensing volume
5-1000µl in 5µl increments, accuracy: ±3µl avg., precision: 5-15µl, <5%, 20-1000µl, <2%. Dispensing speed: 25 sec, 96-well plate, 5µl/well
Catalog #P30016 Luminoskan Ascent Remanufactured