ThermoFisher Luminoskan
Ascent
The
Luminoskan Ascent, combined with powerful PC Software,
handles a wide range of research, industrial and clinical luminescense
applications with 3 year warranty!
-
Plate formats
from 1 to 384 wells
-
Sensitive
-
Dynamic range
- more than 9 decades
-
Optional dispensers - up to 3 can be added
-
Incubation and shaking
-
Powerful PC
Ascent Software included
Instrument Description and
Capabilities: Excellent Flexibility
For maximum versatility and
flexibility, Luminoskan Ascent reads a range of different plate formats,
from 1 to 384-well plates. The high-performance fiberless optics enables
measurement sensitivity of less than 1 fmol of ATP per well, and the dynamic
range of Luminoskan Ascent covers more than nine decades. For easy and
accurate addition of reagents, up to three dispensers can be fitted
on-board. These dispensers allow precise delivery of reagents over an
adjustable volume range of 5 - 1000 µl (not available with this model). For assays requiring sensitive
temperature control, this Luminoskan Ascent has an on-board incubator. Orbital shaking
with adjustable speed and diameter ensures effective mixing and speeds up
reaction times.
Powerful Ascent PC Software
Powerful Software allows
easy assay optimization, flexible data handling and convenient report
formatting. For flash luminescence reactions performed with acridinium
esters or aequorin, for example, software supports simultaneous reagent
injection and signal monitoring. The ability to add reagents and take
readings in any order allows for multiphase ATP and reporter gene assays.
Application areas of the
Luminoskan Ascent
When combined with PC Software,
the Luminoskan Ascent works well for all luminescent applications.
- Reporter gene assays,
such as:
- Eukaryotic luciferase
gene activity
- Prokaryotic bacterial
luciferase gene activity
- Promega
Dual-Luciferase Assay
- Gene Activities with
luminescent substrates
- Immunoassays with
luminescent substrates
- Alkaline phosphatease,
horseradish peroxidase, and others.
- Cytotoxicity and cell
proliferation assays
- MIC, EC, ATP assays
- Growth inhibition
assays
- Intracellular Ca2+ assays
- ATP assays
- DNA quantitation
- Biotin/streptavidin-linked
chemiluminescent assays
- Phagocytosis assays
- Reactive oxygen assays
- Microbiological assays
- Antibiotic sensitivity
testing
- Hygiene monitoring
assays
- Enzyme assay
- Connected assays via
ATP
- Assays with
chemiluminescent substrates
Technical
Specifications:
| Weight |
Basic unit 21kg
(46 lbs). Optional 3
dispenser add 3.5 kg |
| Overall dimensions |
420mm (W) x 420 mm (D) x
340 mm (H) |
| Operating conditions |
+10ºC -- +40ºC, Relative
humidity 90% max. |
| Warm-up time |
<15 min to rated accuracy |
| Detector |
Photomultiplier tube (PMT) |
| Spectral Range |
270-670 nm |
| Measurement range |
0.0001 - 5000 Relative
Light Units (RLU) |
| Gain |
User changeable (300 - 1000
V) |
| Plate types |
1-, 6-, 12-, 24-, 48-, 96-,
and 384-well plates
Maximum dimensions 90 mm x 134 mm x 25 mm. |
| Measuring Speed |
Minimum measurement time
for 96-well plate, 15s |
|
Sensitivity |
10 amol ATP/well using
flash reaction,
white 384-well plate |
| Dynamic range |
>9 decades over whole gain
setting area |
| Shaker |
Orbital
shaker, speed 60 -
1200 RPM, ø 1-50 mm |
| Incubator |
From RT (25ºC) +3ºC to
+45ºC when ambient temperature is 25ºC |
| Dispensers |
Optional 1-3 dispensers.
Dispensing volume
5-1000µl in 5µl increments, accuracy: ±3µl avg., precision: 5-15µl, <5%,
20-1000µl, <2%. Dispensing speed: 25 sec, 96-well plate, 5µl/well |
| Catalog #P30016 |
Luminoskan Ascent Remanufactured |
|
Catalog #P30017 |
Luminoskan Ascent
with one injector Remanufactured |
|
Catalog #P30018 |
Luminoskan Ascent
with two injectors Remanufactured |
|
Catalog #P30019 |
Luminoskan Ascent
with three injectors Remanufactured |
Warranty information:
Comes complete with 110 Voltage line cord
(easily reset to 220 voltage),
manual, technical support and 3 year warranty! Made
in Finland, highest quality instrument.
Easy operation with PC software, accurate and precision
oriented reader.
ThermoFisher Labsystems factory service trained for +10 years.
Microplate Instrumentation
Sales, service and support since 1982.
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