Instrument Description
and Capabilities:
VICTOR 3 multi-task plate reader
designed to support the future demands of industrial and academic
laboratories for multiple assay technologies on a single platform.
Top of the line plate readers for fluorescence, luminescence,
and absorbance measurements. With added injector(s), the Victor
3 performs fill, empty or flush
operations to designated plate.
The 1420 is an all in one reader plate reader capable of reading
Luminescence, Fluorescence, and Absorbance readings. With a complete
platform for quantitative detection of Light-emitting or
light-absorbing markers, this system is suitable for high
sensitivity time-resolved fluorometry, fluorescence, flash or glow
luminescence, flash absorbance and photometry. VICTOR 3 allows
immediate access to more than 10 counting modes, covering all of the
main nonradioactive counting technologies.
VICTOR 3 accepts all types of microtitration plates with between 1
and 864 (1536 fluorescence and time-resolved fluorescence) wells, as
well as Petri dishes, slides, filters and Terasaki plates.
All models include scanning, shaking and kinetics modules. To
support individual applications in cell or molecular biology,
binding studies, environmental and food testing, toxicology and drug
screening, VICTOR 3 can also be supplied with dispenser module,
temperature control, bottom reading, high density reading and
various other options.
A small bench top unit, VICTOR 3 is easy to place as a stand-alone
instrument or to integrate into a robotic system. Performing the
work of a fluorometer, a luminometer, a time-resolved fluorometer
and a photometer, it provides a superior alternative to a whole
series of mutually incompatible analytical instruments.
The VICTOR 3 multilabel counter with IR, high density TR-fluorometry,
stacker (hoppers needed) and robot loading option for fluorescence,
high density time-resolved fluorescence, luminescence and absorption
measurements.
Fluorescence
(340 – 850 nm)
• Dual ratio measurements
• Top and bottom fluorescence
Luminescence
• Glow luminescence
• Flash luminescence
• Dual luminescence |
Photometry (340
– 800 nm)
UV Photometry (260 nm/280 nm)
Time-resolved Fluorometry (TRF)
• Dual window TRF
• Dual emission measurements
Fluorescence Polarization:
(400 – 850 nm) |
A wide range of high quality filters is factory
installed (2 pairs).
These filters cover the most common wavelengths used
in fluorometric and photometric applications, or your choice of
filter pairs. Additional
filters are available upon request.
| Absorbance |
405 nm, 450 nm and 492 nm |
| UV absorbance |
260 nm and 280 nm
|
| Fluorescence |
|
| Excitation |
355 nm and 485 nm |
| Emission |
460 nm and 535 nm |
| TR Fluorescence |
|
| Excitation |
340 nm |
| Emission |
545 nm, 572 nm, 616 nm and 642 nm |
Additional 665 nm emission filter for
LANCE™
comes with red sensitive PMT |
|
| Fluorescence polarization |
|
| Excitation |
480 nm and 531 nm |
| Emission |
535 nm and 595 nm |
| Fluorometry |
|
| Fluorescein |
< 2 fmol/well |
| Linearity |
> 5 decades |
| Crosstalk |
< 0.0001 |
| Umbelliferone |
< 200 fmol/well |
| Rhodamine |
< 100 fmol/well |
| Time Resolved-Fluorometry |
|
| Eu typically |
< 10 amol/well |
| Eu linearity |
> 5 decades |
| Crosstalk |
< 0.0001 |
| Tb typically |
< 5 amol/well |
| Sm typically |
< 50 amol/well |
| Dy typically |
< 150 amol/well |
| Photometry |
340-800nm |
| Measuring range |
@ 405 nm 0 – 2 A |
| Accuracy |
@ 405 nm < 2% or (0.01A) |
| Precision |
@ 405 nm < 0.5% or (0.01A) |
| Luminometry |
|
| Lower limit of detection |
< total flux of 105 photons/sec |
| ALP typically |
1 amol/well with AMPPD substrate |
| ATP typically |
80 amol/well in flash assays |
| Luciferase |
0.9 pg/well using Genglow-100 or GeneLux |
| Fluorescence Polarization |
400-850nm |
| Fluorescein |
1 nM (384-well plate) |
| Standard deviation |
< 5 mP |
| |
|
| Ordering
Information: |
|
Victor 2
stand alone |
Catalog #P97095 (3 in stock) |
Victor 2V
stand alone |
Catalog #P97096 (1 in stock) |
| Victor 2V with 2 injector modules |
Catalog #P97093 (1 in stock) |
| Victor
3 stand alone |
Catalog #P97091 (1 in stock) |
|