| Plate formats |
6 to 1536 well plates |
| Temperature
control |
Ambient + 4 °C to 42 °C
|
| Shaking |
linear, orbital, 3 velocities |
| Fluorescence Intensity |
|
| Wavelength selection |
Monochromator |
| Wavelength range |
Excitation: 230 – 850 nm,
emission: 280 – 850 nm |
| Bandwidth |
Adjustable from 2.5 nm – 10 nm
below 280 nm,
5 nm – 20 nm else |
| Wavelength
accuracy |
± 1 nm |
Wavelength precision |
± 1 nm |
| Sensitivity* –
top reading |
|
| – 384 well plate |
0.2 fmol / well Fluorescein |
| Sensitivity* – bottom reading |
|
| – 384 well plate |
0.6 fmol / well Fluorescein |
| Time Resolved Fluorescence |
|
| Sensitivity* – 384
well plate |
60 amol / well Europium |
| Precision – 384 well
plate |
3 mP standard deviation @ 1nM
Fluorescein |
| Absorbance |
|
| Wavelength range |
230 – 1000 nm |
| Bandwidth |
2.5 nm below 280nm, 5 nm else |
| Wavelength
accuracy |
± 1 nm |
| Wavelength precision |
± 1 nm |
| Measurement range |
0 – 4OD |
| Accuracy @ 492 nm |
0 – 2OD: ± 1% and 0.006OD |
| Precision @
492 nm |
0 – 2OD: ±
0.5% and 0.005OD |
| Fluorescence Absorbance FP |
|
| 384 well plate |
25 sec 25 sec 45
sec |
| 1536 well
plate |
37 sec 27 sec 108
sec |
| Read times – fast scanning |
Fluorescence
scanning of 384 well plate, 450 nm – 550 nm,
5nm step: 321 sec for 384 spectra |
Absorbance scanning of 384 well plate, 450 nm – 550 nm,
5nm step: 103 sec for 384 spectra |
| Interface
connections |
RS 232 serial
& parallel port |
| Tecan Safire Ordering Information |
Catalog #P97088
(2) |
