TECAN ULTRA Microplate Reader, a multi-functional microplate reader developed especially for life scientists working with very high throughputs. The ULTRA lets you choose between several fluorescence techniques as well as absorbance and luminescence modes, without having to change the optical system.

Specifications include:        

• Fully modular system
• Top and bottom Fluorescence Intensity capabilities FI
• Excitation-, Emission-, Fluorescence
• Fluorescence Resonanace Energy Transfer FRET
• Adjustable bandwidth
• Fluorescence Time Resolved TRF
• Fluorescence Polarization FP
• Multichannel Absorbance
• Multichannel Luminescence Glow Type
• 6-1536-well plate capability
• Fast wavelength scanning capabilities
• High sensitivity for short measurement times

With years of TECAN expertise behind it, the ULTRA gives you precise readings every time, even at very low detection limits. This flexible reader can handle any plate format up to 1536 wells, for all techniques. It can read a 1536 well plate in less than a minute and so is ideal for very high throughput applications when you need results immediately.

Three axis positioning (x, y, z) allows you to select the best measuring conditions to suit different well shapes, sample volumes and assay types, giving outstanding sensitivity. Various filters are available for different applications, including special dichroic mirrors for Fluorescein, Europium and Cy5 dyes.

The ULTRA is a fully automatic, computer controlled Fluorescence, Fluorescence Intensity, FRET, TRF, FP, Luminescence and Absorbance instrument for measuring samples in a microplate. The instrument fulfills the requirements for numerous fluorescence, luminescence and absorbance applications in research and routine investigations.

The filters are arranged in filter slides; four excitation filters and four emission filters respectively can be installed in the slides. Handles 6, 24, 96, 384 and 1536 well plates. Spectral range 230-999nm for fluorescence. 230-1000nm absorbance spectral range. Sensitivity ≤1.5 picogram fluorescein on each well or ≤4 fmol well. The emission signal is measured by a highly sensitive PMT. Different detection techniques with one instrument change of measurement directions (top / bottom) by PC software.  Thermal incubation at  +5º C to + 42º C.

Dual optical channels allow measurements from above or below the plate by mouse-click. Light to and from the samples is focused by a lens and high quality filters are easily accessible from the front of the instrument. Each filter slide can hold up to four filters.
Orbital shaking and temperature control are standard.

Suggested use with black plates for bottom-to-top readings. Optimal use with black plates prevent cross-contamination.

 

Plate formats	6 to 1536 well plates
Absorbance Range	230-999nm
Temperature control	Ambient + 5 °C to 42 °C
Shaking	linear or orbital, 3 velocities
Fluorescence Intensity
Wavelength range	Excitation: 230 – 850 nm, emission: 280 – 850 nm
Bandwidth	Adjustable from 2.5 nm – 10 nm below 280 nm, 5 nm – 20 nm else
Wavelength accuracy	± 1 nm
Wavelength precision	± 1 nm
Sensitivity* – top reading
– 384 well plate	0.2 fmol / well Fluorescein
Sensitivity* – bottom reading
– 384 well plate	0.6 fmol / well Fluorescein
Time Resolved Fluorescence
Sensitivity* – 384 well plate	60 amol / well Europium
Fluorescence Polarization
Precision – 384 well plate	3 mP standard deviation @ 1nM Fluorescein
Fluorescence Polarization	Wavelength Range 275-750nm
Absorbance
Wavelength range	230 – 1000 nm
Bandwidth	2.5 nm below 280nm, 5 nm else
Wavelength accuracy	± 1 nm
Wavelength precision	± 1 nm
Measurement range	0 – 4OD
Accuracy @ 492 nm	0 – 2OD: ± 1% and 0.006OD
Precision @ 492 nm	0 – 2OD: ± 0.5% and 0.005OD
Glow Type Luminescence	Chemi or Bioluminescence
Wavelength readings	400-700NM
Fluorescence Absorbance FP
384 well plate	25 sec 25 sec 45 sec
1536 well plate	37 sec 27 sec 108 sec
Read times – fast scanning
Fluorescence scanning of 384 well plate, 450 nm – 550 nm, 5nm step: 321 sec for 384 spectra
Absorbance scanning of 384 well plate, 450 nm – 550 nm, 5nm step: 103 sec for 384 spectra
Interface Connections	RS 232 serial & parallel port
Tecan Ultra Ordering Information	Catalog #P97087 Remanufactured  (1)