Microplate Instrumentation Specialists

Ordering Information
Phone 1.800.848.6474
Phone 01.703.821.1045
Fax 01.703.821.1046
Email info@mtxlsi.com

Labsystems Finstruments Fluorescence|

|Fluoroskan II|

|Fluoroskan I|Accessories|

Ascent Fluorometer and Luminometer

With two instruments in one, the Fluoroskan Ascent FL doubles your value.

Two-in-one fluorometric and luminometric reader for outstanding versatility and efficiency:

State-of-the-art optical system for unrivalled sensitivity
Highly-visual Ascent Software simplifies fluorometric and luminometric applications Ascent FL
On-board precision dispensers (your choice) for rapid kinetic measurements on FL model only
Flexible plate formats, from 1 to 384 wells, enable reading in primary wells both models
Reads from above or below the plate both models
Precise temperature control provides optimum conditions for cell biology and enzyme assays both models
Variable speed orbital shaker for true mixing both models
Simple and effective robotic integration with Assist

Peak performance for fluorometric and luminometric measurements

The Fluoroskan Ascent and the two-in-one Fluoroskan Ascent FL set the standard in performance and ease of use. For even greater flexibility in the lab, the Fluoroskan Ascent FL is equipped with luminometric technology, complementing the long list of advanced features of the Fluoroskan Ascent, which is designed for fluorometric measurements. The Fluoroskan Ascent FL provides full coverage for fluorometric and glow and flash luminometric applications. Together with powerful PC Software, it is a high-performance tool for research, industrial and clinical applications.

The application areas of the Fluoroskan Ascent and Ascent FL models range from cell biology and immunology to molecular biology and microbiology. For many applications - including Ca²⁺ measurement, cell proliferation, cytotoxicity, hybridization assays, reporter gene assays, phagocytosis and immunoassays - both fluorometric and luminometric detection methods can be used.

Flexibility plus lower costs

The Fluoroskan Ascent FL doubles your value - reducing costs, improving efficiency and saving bench space in the lab. These benefits also apply to robot-controlled applications because just one system - hardware and software - covers both fluorometric and luminometric techniques. Even during a single session, the Fluoroskan Ascent FL provides the freedom to perform both types of measurements.

High-performance optics

The direct illumination optics of the Fluoroskan Ascent FL produces a highly focused light beam. In fluorescence measurement, this prevents crosstalk and ensures accurate readings. Two beam diameter settings, 3 mm and 1.5 mm, allow optimal readings of
1-96 and 384-well plates. Automatic gain selection eliminates the need for manual adjustment in fluorometry. It also guarantees the best possible sensitivity and dynamic range for all wavelengths. High sensitivity is one of the main benefits of fibreless optics -- and a critical feature for luminometry.

Market-leading dispenser system

For easy and accurate addition of reagents, up to three dispensers can be fitted on board the Fluoroskan Ascent and Ascent FL. Dispensing volumes can be adjusted in
5µl increments over a volume range of 5-1000µl.

Speed and precision are the key features of these dispensers. The system can dispense and measure simultaneously, which enables Ca²⁺ measurement and other rapid kinetic applications. The fast read time of the Fluoroskan Ascent FL, just 15 seconds for a 96-well plate, is another important advantage for kinetic applications.

On-board incubation and orbital shaking

An incubator option is available for cell biology, enzyme assays and other applications requiring precise temperature control.

Built-in orbital shaking, another advanced feature, speeds up reaction times and ensures effective mixing. Both the speed and amplitude of shaking can be adjusted.

Effective robotic integration

Robotic integration is simple and effective with the Fluoroskan Ascent and Ascent FL. The plate carrier allows convenient access for the robotic arm, and Ascent Software is easy to integrate with robotic systems. The execution of predefined assays can be controlled remotely, and the software can be set up to export and import data automatically.

The instrument is supplied with PC software and one filter pair of users choice.

Technical Specifications:

Plate types:	1-, 6-, 12-, 24-, 48-, 96-, and 384-well plates Can also be programmed for custom configurations. Maximum dimensions 90 mm x 134 mm x 25 mm.

Optical Performance:
Light source:	Quartz halogen lamp in stock
Detector:	Photomultiplier tube
Excitation wavelength range:	From 320 nm to 620 nm
Emission wavelength range:	From 360 nm to 670 nm
Filters:	High-quality interference filters. The excitation filter wheel can hold a maximum of eight filters, and the emission filter wheel can hold a maximum of six filters.
Excitation filters *) standard	320 nm, 355 nm, 390 nm, 444 nm, 485 nm, 530 nm, 544 nm, 584 nm. your custom filters available upon request.
Emissions filters *) standard	405 nm, 460 nm, 485 nm, 510 nm, 538 nm, 555 nm, 590 nm, 612 nm, 620 nm. your custom filters available upon request.
*)Standard instrument includes 1 filter pair of choice.

Performance Specifications
Fluoroskan Fluorometer
Theoretical sensitivity:	2 fmol fluorescein/well in a black 96-well plate
Dynamic Range:	> 5 decades
Luminoskan Luminometer
Theoretical sensitivity:	5 fmol ATP / well in a white 96-well plate (ATP Monitoring kit)
Dynamic range:	7 decades

Shaker:	Orbital

Dispensers:	1 to 3 dispensers (as needed)
Dispensing volume:	5-1000 µl in 5 µl increments
Accuracy:	±3 µl average
Precision:	5-15 µl < 5%, 20-1000 µl < 2%
Dispensing speed:	25 seconds per plate (96-well plate, 5µl/well)

Incubator:
Temperature range:	From RT +3ºC to +45ºC

P21025	Fluoroskan Ascent FL NEW
P21024	Fluoroskan Ascent FL Demo
P21026	Fluoroskan Ascent FL one dispenser
P21027/P21030	Fluoroskan Ascent FL 2 or 3 dispensers
Fluorescence Filters and Applications	click here!

Robotic Interface