instruments in one, the Fluoroskan Ascent FL doubles your value.
Two-in-one fluorometric and
luminometric reader for outstanding versatility and efficiency:
- State-of-the-art optical
system for unrivalled sensitivity
- Highly-visual Ascent
Software simplifies fluorometric and luminometric applications Ascent FL
- On-board precision
dispensers (various models) for rapid kinetic measurements on FL model only
- Flexible plate formats,
from 1 to 384 wells, enable reading in primary wells both models
- Reads from above or below
the plate both models
- Precise temperature control provides
optimum conditions for cell biology and enzyme assays both models
- Variable speed orbital
shaker for true mixing both models
Peak performance for
fluorometric and luminometric measurements
The Fluoroskan Ascent and
the two-in-one Fluoroskan Ascent FL set the standard in performance and ease
of use. For even greater flexibility in the lab, the Fluoroskan Ascent FL is
equipped with luminometric technology, complementing the long list of
advanced features of the Fluoroskan Ascent, which is designed for
fluorometric measurements. The Fluoroskan Ascent FL provides full coverage
for fluorometric and glow and flash luminometric applications. Together with
powerful PC Software, it is a high-performance tool for research,
industrial and clinical applications.
The application areas of the
Fluoroskan Ascent and Ascent FL models range from cell biology and immunology to molecular
biology and microbiology. For many applications - including Ca2+ measurement, cell proliferation, cytotoxicity, hybridization assays,
reporter gene assays, phagocytosis and immunoassays - both fluorometric and
luminometric detection methods can be used.
Flexibility plus lower
The Fluoroskan Ascent FL
doubles your value - reducing costs, improving efficiency and saving bench
space in the lab. These benefits also apply to robot-controlled applications
because just one system - hardware and software - covers both fluorometric
and luminometric techniques. Even during a single session, the Fluoroskan
Ascent FL provides the freedom to perform both types of measurements.
The direct illumination
optics of the Fluoroskan Ascent FL produces a highly focused light beam. In
fluorescence measurement, this prevents crosstalk and ensures accurate
readings. Two beam diameter settings, 3 mm and 1.5 mm, allow optimal
1-96 and 384-well plates. Automatic gain selection eliminates the need for
manual adjustment in fluorometry. It also guarantees the best possible
sensitivity and dynamic range for all wavelengths. High sensitivity is one
of the main benefits of fibreless optics -- and a critical feature for
For easy and accurate
addition of reagents, up to three dispensers can be fitted on board the
Fluoroskan Ascent and Ascent FL. Dispensing volumes can be adjusted in
5µl increments over a volume range of 5-1000µl.
Speed and precision are the
key features of these dispensers. The system can dispense and measure
simultaneously, which enables Ca2+ measurement and other rapid
kinetic applications. The fast read time of the Fluoroskan Ascent FL, just
15 seconds for a 96-well plate, is another important advantage for kinetic
On-board incubation and
An incubator option is
available for cell biology, enzyme assays and other applications requiring
precise temperature control.
Built-in orbital shaking,
another advanced feature, speeds up reaction times and ensures effective
mixing. Both the speed and amplitude of shaking can be adjusted.
Instruments come with 2 filter pairs of choice, line cord and three year warranty from
Factory Trained Engineers.
Additional filter pairs can be purchased.
||1-, 6-, 12-, 24-, 48-, 96-,
and 384-well plates
Can also be programmed for custom configurations.
Maximum dimensions 90 mm x 134 mm x 25 mm.
||Quartz halogen lamp in
||From 320 nm to 620 nm
|Emission wavelength range:
||From 360 nm to 670 nm
filters. The excitation filter wheel can hold a maximum of eight
filters, and the emission filter wheel can hold a maximum of six
|Excitation filters *)
||320 nm, 355 nm, 390 nm, 444
nm, 485 nm,
530 nm, 544 nm, 584 nm.
filters available upon request.
|Emissions filters *)
||405 nm, 460 nm, 485 nm, 510
nm, 538 nm,
555 nm, 590 nm, 612 nm, 620 nm.
filters available upon request.
includes 2 filter pairs of choice.
||2 fmol fluorescein/well in
a black 96-well plate
||> 5 decades
||5 fmol ATP / well in a
white 96-well plate
(ATP Monitoring kit)
||1 to 3 dispensers (optional)
||5-1000 µl in 5 µl
||±3 µl average
||5-15 µl < 5%, 20-1000 µl <
||25 seconds per plate
(96-well plate, 5µl/well)
||From RT +3ºC to +45ºC (optional)
| Interface Connections:
||RS 232 serial
& parallel port
Catalog # P21024
||Fluoroskan Ascent FL
Catalog # P21026
||Fluoroskan Ascent FL with one
Catalog # P21027
||Fluoroskan Ascent FL with
Catalog # P21029
Ascent FL with three