Instrument Description
and Capabilities:
The Synergy 2 is BioTek's high-performance multi-mode plate reader designed for the life science research and drug discovery markets. Its fluorescence detection system uses deep blocking filters and dichroic mirrors for the best level of performance in research and screening applications. The dedicated absorbance detection system is monochromator-based, providing the best level of flexibility in this read mode. And, by utilizing dedicated optical elements for each individual detection technique, Synergy 2 offers uncompromised performance and the lowest limits of detection.
Detection modes include Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescence, AlphaScreen, Luminescence and UV-visible Absorbance. Its three broad-spectrum light sources have been chosen for optimal illumination and excitation in all applications.
The Synergy 2 is controlled by BioTek's Gen5™ Reader Control and Data Analysis Software, is certified for HTRF®, DLR™ and LanthaScreen™, and is compatible with AlphaScreen assays.
|
Specifications: |
| Detection method |
Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa, Luminescence, UV-Visible Absorbance |
| Read method |
End-point, kinetic, spectral scanning, well-area scanning |
| Microplate types |
6- to 1536-well plates (luminescence 1-384)
PCR plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots |
| Temperature control |
4°C above ambient to 65°C |
| Shaking |
Included |
|
PC Software |
Gen5™
to be purchased
Control through USB or serial port |
| Absorbance |
| Light source |
SQ Xenon Flash |
| Wavelength selection |
Monochromator |
| Wavelength range |
200 - 999 nm, 1 nm increment |
| Bandpass |
2.4 nm |
| Dynamic range |
0 - 4.0 OD |
| Resolution |
0.0001 OD |
| Pathlength correction |
Yes |
| Monochromator wavelength accuracy |
+/- 2 nm |
| Monochromator wavelength repeatability |
+/- 0.2 nm |
| OD accuracy |
< 1% at 2 OD typical
< 3% at 3.0 OD typical |
| OD linearity |
< 1% from 0 to 3.0 OD typical |
| OD repeatability |
< 0.5% at 2.0 OD typical |
| Stray light |
0.03% at 230 nm typical |
| Reading speed |
96: 11 seconds
384: 22 seconds
1536: 42 seconds |
| Fluorescence Intensity |
| Sensitivity |
Fluorescein 1 pM typical (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate) - Top
Fluorescein 5 pM typical (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate) - Bottom |
| Light source |
Tungsten Halogen High Energy DPR Xenon Flash (optional) |
| Wavelength selection |
Deep blocking bandpass filters/dichroic mirrors |
| Wavelength range |
300 - 700 nm with tungsten lamp (900 nm option)
200 - 700 nm with xenon lamp (900 nm option) |
| Dynamic range |
> 6 decades |
| Bandpass |
Filter dependent |
| Detection system |
PMT |
| Reading speed |
96: 11 seconds
384: 22 seconds
1536: 42 seconds |
| Luminescence |
| Sensitivity |
10 amol ATP typical (flash) |
| Wavelength range |
300 - 700 nm |
| Dynamic range |
> 6 decades |
| Detection system |
Low noise PMT |
| Fluorescence Polarization |
| Light source |
Tungsten Halogen High Energy DPR Xenon Flash (optional) |
| Sensitivity |
3 mP at 1 nM fluorescein typical |
| Wavelength selection |
Deep blocking filters/mirrors |
| Wavelength range |
400 - 700 nm |
| Time-Resolved Fluorescence |
| Light source |
High Energy DPR Xenon Flash |
| Sensitivity |
Europium 60 fM typical (12 amol/well 96-well plate; 6 amol/well 384-well plate) |
| Wavelength selection |
Deep blocking filters/mirrors |
| Wavelength range |
200 - 700 nM (900 nm option) |
| Reagent Dispensers |
| Number |
2 syringe pumps
(option) |
| Dispense volume |
5 - 1000 µL in 1 µL increment |
| Dead volume |
1.1 mL, 100 µL with backflush |
| Physical Characteristics |
| Dimensions |
17"W x 17.5"D x 14.5"H (43.5 x 44.5 x 37.3 cm) |
| Weight |
60 lbs (27 kg) |
|
Catalog # |
P97238 |
Warranty
information:
Instruments come complete with NIST certification and documentation, line cord and six days a week technical
support. Remanufactured model
with one year warranty. High quality instrument made in USA. Easily reset to 220 Volt.
Microplate Instrumentation
Sales, Service and Support since 1982. |
