| Fluorochrome |
Excitation Wavelength |
Emission Wavelength |
| Acid
Fuchsin |
| 540 |
 |
|
|
| 630 |
|
|
|
Acridine Orange
(Bound to DNA) |
| 502 |
|
|
|
| 526 |
|
|
|
| Acridine Red |
|
|
| Acridine Yellow |
| 470 |
|
|
|
| 550 |
|
|
|
| Acriflavin |
| 436 |
|
|
|
| 520 |
|
|
|
AFA
(Acriflavin
Feulgen SITSA) |
|
| 460 |
|
|
|
| Alizarin Complexon |
|
| 580 |
|
|
|
| Alizarin Red |
|
| 580 |
|
|
|
| Allophycocyanin |
| 650 |
|
|
|
| 661 |
|
|
|
| ACMA |
| 430 |
|
|
|
| 474 |
|
|
|
| AMCA-S,
AMC |
| 345 |
|
|
|
| 445 |
|
|
|
| Aminoactinomycin D |
| 555 |
|
|
|
| 655 |
|
|
|
| 7-Aminoactinomycin D-AAD |
| 546 |
|
|
|
| 647 |
|
|
|
| Aminocoumarin |
| 350 |
|
|
|
| 445 |
|
|
|
| Anthroyl Stearate |
|
| 446 |
|
|
|
Astrazon Brilliant
Red 4G |
| 500 |
|
|
|
| 585 |
|
|
|
| Astrazon Orange R |
| 470 |
|
|
|
| 540 |
|
|
|
| Astrazon Red 6B |
| 520 |
|
|
|
| 595 |
|
|
|
Astrazon Yellow
7 GLL |
| 450 |
|
|
|
| 480 |
|
|
|
| Atabrine |
| 436 |
|
|
|
| 490 |
|
|
|
| Auramine |
| 460 |
|
|
|
| 550 |
|
|
|
| Aurophosphine |
|
| 515 |
|
|
|
| Aurophosphine G |
| 450 |
|
|
|
| 580 |
|
|
|
BAO
9-(Bisamino-
phenyloxadiazole) |
| 365 |
|
|
|
| 395 |
|
|
|
| BCECF |
| 505 |
|
|
|
| 530 |
|
|
|
| Berberine Sulphate |
| 430 |
|
|
|
| 550 |
|
|
|
| Bisbenzamide |
| 360 |
|
|
|
|
| BOBO-1, BO-PRO-1 |
| 462 |
|
|
|
| 481 |
|
|
|
| Blancophor FFG Solution |
| 390 |
|
|
|
| 470 |
|
|
|
| Blancophor SV |
| 370 |
|
|
|
| 435 |
|
|
|
| Bodipy Fl |
| 503 |
|
|
|
| 512 |
|
|
|
| Bodipy TMR |
| 542 |
|
|
|
| 574 |
|
|
|
| Bodipy TR |
| 589 |
|
|
|
| 617 |
|
|
|
| BOPRO 1 |
| 462 |
|
|
|
| 481 |
|
|
|
Brilliant Sulpho-
flavin FF |
| 430 |
|
|
|
| 520 |
|
|
|
| Calcein |
| 494 |
|
|
|
| 517 |
|
|
|
| Calcein Blue |
| 370 |
|
|
|
| 435 |
|
|
|
| Calcium Green |
| 505 |
|
|
|
| 532 |
|
|
|
| Calcium Orange |
| 549 |
|
|
|
| 576 |
|
|
|
| Calcofluor RW Solution |
| 370 |
|
|
|
| 440 |
|
|
|
| Calcofluor White |
| 440 |
|
|
|
|
Calcofluor White
ABT Solution |
| 380 |
|
|
|
| 475 |
|
|
|
Calcofluor White
Standard Solution |
| 365 |
|
|
|
| 435 |
|
|
|
5-(and 6-)carboxy
SNARF-1 indicator |
| 548 (Low pH) |
|
|
| 576 (High pH) |
|
|
|
| 587 (Low pH) |
|
|
| 635 (High pH) |
|
|
|
| 6-Carboxyrhodamine 6G |
| 525 |
|
|
|
| 555 |
|
|
|
| Cascade Blue |
| 400 |
|
|
|
| 425 |
|
|
|
| Catecholamine |
| 410 |
|
|
|
| 470 |
|
|
|
| Chinacrine |
|
| 515 |
|
|
|
| CL-NERF |
| 504 (Low pH) |
|
|
| 514 (High pH) |
|
|
|
| 587 (Low pH) |
|
|
| 540 (High pH) |
|
|
|
| Coriphosphine O |
| 460 |
|
|
|
| 575 |
|
|
|
| Coumarin-Phalloidin |
| 387 |
|
|
|
| 470 |
|
|
|
| CY3.18 |
| 554 |
|
|
|
| 568 |
|
|
|
| CY5.18 |
| 649 |
|
|
|
| 666 |
|
|
|
| CY7 |
| 710 |
|
|
|
| 805 |
|
|
|
DANS
(1-DimethylAmino-
Naphthaline-5-Sulphonic Acid) |
| 340 |
|
|
|
| 525 |
|
|
|
DANSA
(DiaminoNaphthyl-
Sulphonic Acid) |
|
| 430 |
|
|
|
| Dansyl NH-CH3 in water |
| 340 |
|
|
|
| 578 |
|
|
|
| DAPI |
| 350 |
|
|
|
| 470 |
|
|
|
| DiA |
| 456 |
|
|
|
| 590 |
|
|
|
Diamino Phenyl
Oxydiazole (DAO) |
| 280 |
|
|
|
| 460 |
|
|
|
| Di-8-ANEPPS |
| 488 |
|
|
|
| 605 |
|
|
|
Dimethylamino-5-
Sulphonic Acid |
|
| 520 |
|
|
|
| DiI
[DiIC18(3)] |
| 549 |
|
|
|
| 565 |
|
|
|
| DiO
[DiOC18(3)] |
| 484 |
|
|
|
| 501 |
|
|
|
Diphenyl Brilliant
Flavine 7GFF |
| 430 |
|
|
|
| 520 |
|
|
|
| DM-NERF |
| 497 (Low pH) |
|
|
| 510 (High pH) |
|
|
|
| 527 (Low pH) |
|
|
| 536 (High pH) |
|
|
|
| Dopamine |
| 340 |
|
|
|
|
| ELF-97 alcohol |
| 345 |
|
|
|
| 530 |
|
|
|
| Eosin |
| 525 |
|
|
|
| 545 |
|
|
|
| Erythrosin ITC |
| 530 |
|
|
|
| 558 |
|
|
|
| Ethidium Bromide |
| 510 |
|
|
|
| 595 |
|
|
|
| Euchrysin |
| 430 |
|
|
|
| 540 |
|
|
|
FIF
(Formaldehyde
Induced Fluorescence) |
| 405 |
|
|
|
| 435 |
|
|
|
| FITC |
| 480 |
|
|
|
535
|
| Flazo Orange |
|
| 612 |
|
|
|
| Fluorescein |
| 494 |
|
|
|
| 518 |
|
|
|
Fluorescein Iso-
thiocyanate (FITC) |
| 490 |
|
|
|
| 525 |
|
|
|
| Fluo
3 |
| 485 |
|
|
|
| 503 |
|
|
|
| FM1-43 |
| 479 |
|
|
|
| 598 |
|
|
|
| Fura-2 |
| 363 (Low [Ca2+]) |
|
|
|
| 335 (High [Ca2+]) |
|
|
|
|
| 512 (Low [Ca2+]) |
|
|
|
| 505 (High [Ca2+]) |
|
|
|
|
| Fura
Red |
| 472 (Low [Ca2+]) |
|
|
|
| 436 (High [Ca2+]) |
|
|
|
|
| 657 (Low [Ca2+]) |
|
|
|
| 637 (High [Ca2+]) |
|
|
|
|
Genacryl Brilliant
Red B |
| 520 |
|
|
|
| 590 |
|
|
|
Genacryl Brilliant
Yellow 10GF |
| 430 |
|
|
|
| 485 |
|
|
|
| Genacryl Pink 3G |
| 470 |
|
|
|
| 583 |
|
|
|
Genacryl Yellow
5GF |
| 430 |
|
|
|
| 475 |
|
|
|
| Gloxalic Acid |
| 405 |
|
|
|
| 460 |
|
|
|
| Granular Blue |
| 355 |
|
|
|
| 425 |
|
|
|
| Haematoporphyrin |
|
| 580 |
|
|
|
Hoechst 33258, 33342
(Bound to DNA) |
| 352 |
|
|
|
| 461 |
|
|
|
3-Hydroxypyrene-5,-
8,10-TriSulfonic Acid |
| 403 |
|
|
|
| 513 |
|
|
|
7-Hydroxy-4-
methylcoumarin |
| 360 |
|
|
|
| 455 |
|
|
|
5-Hydroxy-
Tryptamine (5-HT) |
|
|
| Indo-1 |
| 350 |
|
|
|
|
Intrawhite Cf
Liquid |
| 360 |
|
|
|
| 430 |
|
|
|
| Leucophor PAF |
| 370 |
|
|
|
| 430 |
|
|
|
| Leucophor SF |
| 380 |
|
|
|
| 465 |
|
|
|
| Leucophor WS |
| 395 |
|
|
|
| 465 |
|
|
|
Lissamine Rhodamine
B200 (RD200) |
| 575 |
|
|
|
| 595 |
|
|
|
| Lucifer Yellow CH |
| 425 |
|
|
|
| 528 |
|
|
|
| Lucifer Yellow VS |
| 430 |
|
|
|
| 535 |
|
|
|
LysoSensor Blue
DND-192, DND-167 |
| 374 |
|
|
|
| 425 |
|
|
|
LysoSensor Green
DND-153, DND-189 |
| 442 |
|
|
|
| 505 |
|
|
|
| LysoSensor Yellow/Blue |
| 384 (Low pH) |
|
|
| 329 (High pH) |
|
|
|
| 540 (Low pH) |
|
|
| 440 (High pH) |
|
|
|
| LysoTracker Green |
| 504 |
|
|
|
| 511 |
|
|
|
| LysoTracker Yellow |
| 534 |
|
|
|
| 551 |
|
|
|
| LysoTracker Red |
| 577 |
|
|
|
| 592 |
|
|
|
| Magdala Red |
| 524 |
|
|
|
| 600 |
|
|
|
| Magnesium Green |
| 506 |
|
|
|
| 531 |
|
|
|
| Magnesium Orange |
| 550 |
|
|
|
| 575 |
|
|
|
Maxilon Brilliant
Flavin 10 GFF |
| 450 |
|
|
|
| 495 |
|
|
|
Maxilon Brilliant
Flavin 8 GFF |
| 460 |
|
|
|
| 495 |
|
|
|
| Mitotracker Green FM |
| 490 |
|
|
|
| 516 |
|
|
|
Mitotracker Orange
CMTMRos |
| 551 |
|
|
|
| 576 |
|
|
|
MPS
(Methyl Green
Pyronine Stilbene) |
| 364 |
|
|
|
| 395 |
|
|
|
| Mithramycin |
| 450 |
|
|
|
| 570 |
|
|
|
| NBD |
| 465 |
|
|
|
| 535 |
|
|
|
| NBD
Amine |
| 450 |
|
|
|
| 530 |
|
|
|
| Nile
Red |
|
|
| Nitrobenzoxadidole |
|
|
| Noradrenaline |
| 340 |
|
|
|
|
| Nuclear Fast Red |
|
| 580 |
|
|
|
| Nuclear Yellow |
| 365 |
|
|
|
| 495 |
|
|
|
Nylosan Brilliant
Flavin E8G |
| 460 |
|
|
|
| 510 |
|
|
|
Oregon Green 488
fluorophore |
| 496 |
|
|
|
| 524 |
|
|
|
Oregon Green 500
fluorophore |
| 503 |
|
|
|
| 522 |
|
|
|
Oregon Green 514
fluorophore |
| 511 |
|
|
|
| 530 |
|
|
|
Pararosaniline
(Feulgen) |
| 570 |
|
|
|
| 625 |
|
|
|
Phorwite AR
Solution |
| 360 |
|
|
|
| 430 |
|
|
|
| Phorwite BKL |
| 370 |
|
|
|
| 430 |
|
|
|
| Phorwite Rev |
| 380 |
|
|
|
| 430 |
|
|
|
| Phorwite RPA |
| 375 |
|
|
|
| 430 |
|
|
|
| Phosphine 3R |
| 465 |
|
|
|
| 565 |
|
|
|
| Phosphine R |
|
| 578 |
|
|
|
Pontochrome
Blue Black |
|
| 605 |
|
|
|
| POPO-1, PO-PRO-1 |
| 434 |
|
|
|
| 456 |
|
|
|
| Primuline |
| 410 |
|
|
|
| 550 |
|
|
|
| Procion Yellow |
| 470 |
|
|
|
| 600 |
|
|
|
| Propidium Iodide |
| 536 |
|
|
|
| 617 |
|
|
|
| Pyronine |
| 410 |
|
|
|
| 540 |
|
|
|
| Pyronine B |
|
|
Pyrozal Brilliant
Flavin 7GF |
| 365 |
|
|
|
| 495 |
|
|
|
Quinacrine
Mustard |
| 423 |
|
|
|
| 503 |
|
|
|
| R-phycoerythrin |
| 565 |
|
|
|
| 575 |
|
|
|
| Rhodamine 110 |
| 496 |
|
|
|
| 520 |
|
|
|
| Rhodamine 123 |
| 511 |
|
|
|
| 534 |
|
|
|
| Rhodamine 5 GLD |
| 470 |
|
|
|
| 565 |
|
|
|
| Rhodamine 6G |
| 526 |
|
|
|
| 555 |
|
|
|
| Rhodamine B |
| 540 |
|
|
|
| 625 |
|
|
|
| Rhodamine B 200 |
|
| 595 |
|
|
|
| Rhodamine B Extra |
| 550 |
|
|
|
| 605 |
|
|
|
| Rhodamine BB |
| 540 |
|
|
|
| 580 |
|
|
|
| Rhodamine BG |
| 540 |
|
|
|
| 572 |
|
|
|
Rhodamine Green
fluorophore |
| 502 |
|
|
|
| 527 |
|
|
|
| Rhodamine Red |
| 570 |
|
|
|
| 590 |
|
|
|
| Rhodamine WT |
| 530 |
|
|
|
| 555 |
|
|
|
| Rhodol Green fluorophore |
| 499 |
|
|
|
| 525 |
|
|
|
| Rose
Bengal |
| 540 |
|
|
|
|
| Serotonin |
| 365 |
|
|
|
|
Sevron Brilliant
Red 2B |
| 520 |
|
|
|
| 595 |
|
|
|
Sevron Brilliant
Red 4G |
| 500 |
|
|
|
| 583 |
|
|
|
Sevron Brilliant
Red B |
| 530 |
|
|
|
| 590 |
|
|
|
| Sevron Orange |
| 440 |
|
|
|
| 530 |
|
|
|
| Sevron Yellow L |
| 430 |
|
|
|
| 490 |
|
|
|
| SITS
(Primuline) |
|
| 450 |
|
|
|
SITS
(Stilbene
Isothiosulphonic Acid) |
| 365 |
|
|
|
| 460 |
|
|
|
| Sodium Green |
| 507 |
|
|
|
| 535 |
|
|
|
| Stilbene |
| 335 |
|
|
|
| 440 |
|
|
|
| Snarf 1 |
| 563 |
|
|
|
| 639 |
|
|
|
Sulpho Rhodamine
B Can C |
| 520 |
|
|
|
| 595 |
|
|
|
Sulpho Rhodamine
G Extra |
| 470 |
|
|
|
| 570 |
|
|
|
SYTOX Green nucleic
acid stain |
| 504 |
|
|
|
| 523 |
|
|
|
SYTO
Green fluorescent
nucleic acid stains |
| 494 ± 6 |
|
|
|
| 515 ± 7 |
|
|
|
SYTO
Green fluorescent
nucleic acid stains |
| 515 ± 7 |
|
|
|
| 543 ± 13 |
|
|
|
SYTO
17 red fluorescent
nucleic acid stain |
| 621 |
|
|
|
| 634 |
|
|
|
| Tetracycline |
| 390 |
|
|
|
| 560 |
|
|
|
TRITC (Tetramethyl
Rhodamine Isothiocyanate) |
| 557 |
|
|
|
| 576 |
|
|
|
| Texas Red |
| 596 |
|
|
|
| 615 |
|
|
|
| Thiazine Red R |
| 510 |
|
|
|
| 580 |
|
|
|
| Thioflavin S |
| 430 |
|
|
|
| 550 |
|
|
|
| Thioflavin TCN |
| 350 |
|
|
|
| 460 |
|
|
|
| Thioflavin 5 |
| 430 |
|
|
|
| 550 |
|
|
|
| Thiolyte |
|
|
| Thiozol Orange |
| 453 |
|
|
|
| 480 |
|
|
|
| Tinopol CBS |
| 390 |
|
|
|
| 430 |
|
|
|
| TOTO
1, TO-RRO-1 |
| 514 |
|
|
|
| 533 |
|
|
|
| TOTO
3, TO-PRO-3 |
| 642 |
|
|
|
| 661 |
|
|
|
| True
Blue |
| 365 |
|
|
|
|
| Ultralite |
| 656 |
|
|
|
| 678 |
|
|
|
| Uranine B |
| 420 |
|
|
|
| 520 |
|
|
|
| Uvitex SFC |
| 365 |
|
|
|
| 435 |
|
|
|
| X-Rhodamine |
| 580 |
|
|
|
| 605 |
|
|
|
| Xylene Orange |
| 546 |
|
|
|
| 580 |
|
|
|
| XRITC |
| 582 |
|
|
|
| 601 |
|
|
|
| YOYO-1, YOYO-PRO-1 |
| 491 |
|
|
|
| 509 |
|
|
|
| YOYO-3, YOYO-PRO-3 |
| 612 |
|
|
|
| 631 |
|
|
|
