Molecular Devices Fluorescence Monochromatic Gemini

The Gemini spectrofluorometer allows for fast, high-sensitivity measurements. Cellular and molecular binding studies, DNA quantitation, drug screening, and SNP genotyping analysis all require a high degree of sensitivity. The Gemini system offers a flexible, filter-free environment allowing the determination of optimal excitation and emission settings in 1.0 nm increments providing the best level of sensitivity for variable assay conditions.

Fluorescence
Time Resolved Fluorescence TRF
Fluorescence Intensity top and bottom capabilities FI
Fluorescence Resonanace Energy Transfer FRET
Temperature control and microplate-shaking included
Adjustable bandwidth range
6-384-well plate capability

Applications

		Cellular and molecular binding studies
		Nucleic acid quantitation
		Cytoxicity assays
		SNP genotype detection and molecular beacons
		Enzyme activity
		CatchPoint^® cAMP Assay Kit

Features

		Dual Monochromators — The right pair of excitation and emission wavelengths is always available because the dual monochromators allow the selection of any wavelength in 1 nm increments. New fluorophores can easily be evaluated without purchasing additional filters.
		Wavelength Scanning — The most sensitive results are achieved by using optimal excitation and emission wavelengths. Literature wavelengths are often based on results from wavelength-limited, filter-based readers. Wavelength scanning ensures that the most sensitive assay conditions are used.
		Well Scanning — Gemini can report a single point from the well center, or multiple data points from large well tissue culture plates. This provides high sensitivity for cell-based assays.

SoftMax Pro software

SoftMax Pro allows you to select excitation and emission values and simply input their values for the monochromator settings. You can easily scan both excitation and emission spectra and determine the optimal settings.

Engineered for superior performance and reliability

Gemini dual monochromator design — an excitation monochromator (A) and emission monochromator (B) — allows user-defined variable wavelength settings. Sending light through a movable scanning grating (D) which selects the desired excitation wavelength. The light is passed through 1 mm fiber optic bundles (E) to elliptical focusing mirrors (F) that maximize light collection efficiency for superior sensitivity and send the beam to a microplate well (G). If fluorescent molecules are present, the light of the emission wavelength is emitted back out to focusing mirrors (H) that direct the light through 4 mm fiber optic bundles (J) to the emission scanning grating (K). The desired wavelength of light is then detected by the photomultiplier tube, or PMT (L). The PMT passes the fluorescence signal through the Gemini’s electronics, creating the signals that result in data points collected by software.

Specifications**

	Fluorescence photometric performance
Dual Monochromators	1 nm increment selection
EX Wavelength	250-850 nm
EM Wavelength	360-850 nm
Bandwidth (Excitation & Emission)	9 nm
Detection Limit (Signal 3X SD of Baseline)	2.0 fmol/well FITC 200 µL in 96 wells
Catalog # 6723117	Softmax Pro PC/MAC Software via interface connections RS 232 serial & parallel port
Catalog # 6723119	SpectraTest ABS 1 validation package
Catalog # P97038	Fully remanufactured with one year warranty

Applications

Features

SoftMax Pro software SoftMax Pro allows you to select excitation and emission values and simply input their values for the monochromator settings. You can easily scan both excitation and emission spectra and determine the optimal settings.

Engineered for superior performance and reliability

Specifications**

Fluorescence photometric performance

SoftMax Pro software

SoftMax Pro allows you to select excitation and emission values and simply input their values for the monochromator settings. You can easily scan both excitation and emission spectra and determine the optimal settings.