Additional in-stock
Multi-Detection and Fluorescence Plate Readers:
|Fluoroskan I|Perseptive Biosystems Cytofluor 4000|BMG
FLUOstar|
|Biotek
FL 600|
|ThermoFisher Finstruments Fluorescence|ThermoFisher
Ascent|
|Tecan
Spectrafluor|Tecan
Spectrafluor Plus|Tecan
Genios|
|Molecular
Devices Gemini XS|
|BMG
FLUOstar Galaxy|Perkin
Elmer Victor 2 with or w/o injectors|
|Thermo Fisher
Ascent FL with w/o injectors|
|Molecular
Devices Gemini EM|
Molecular Devices Spectramax Gemini XS Monochromatic
Fluorescence including FI, FP, TRF and Luminescence
Equipped with UV-VIS Fluorescence
for top- and
bottom- read assays
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The Gemini XS spectrofluorometer allows
for fast, high-sensitivity measurements. Cellular and molecular
binding studies, DNA quantitation, drug screening, and SNP
genotyping analysis all require a high degree of sensitivity. The
Gemini system offers a flexible, filter-free environment
allowing the determination of optimal excitation and emission
settings in 1.0 nm increments providing the best level of
sensitivity for variable assay conditions.
- Fluorescence
- Time Resolved Fluorescence
TRF
- Fluorescence Intensity top
and bottom capabilities FI
- Fluorescence Resonanace Energy
Transfer FRET
- Fluorescence Polarization FP
- Fluorescence filters included
in 1nm range
- Absorbance reading mode (UV and VIS
range)
- Absorbance filters included
in 1 nm range
- Temperature control and microplate-shaking included
- Adjustable bandwidth range
- Luminescence glow reading mode
- 6-384-well plate capability
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Applications
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Cellular
and molecular binding studies |
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Nucleic
acid quantitation |
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Cytoxicity
assays |
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SNP
genotype detection and molecular beacons |
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Enzyme
activity |
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CatchPoint® cAMP Assay Kit |
Features
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Dual
Monochromators — The right pair
of excitation and emission wavelengths is always available
because the dual monochromators allow the selection of any
wavelength in 1 nm increments. New fluorophores can easily be
evaluated without purchasing additional filters. |
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Wavelength
Scanning — The most sensitive
results are achieved by using optimal excitation and emission
wavelengths. Literature wavelengths are often based on results
from wavelength-limited, filter-based readers. Wavelength
scanning ensures that the most sensitive assay conditions are
used. |
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Well
Scanning — Gemini XS can report
a single point from the well center, or multiple data points
from large well tissue culture plates. This provides high
sensitivity for cell-based assays. |
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Auto PMT
Gain — Because a single
microplate often presents a range of fluorescence intensities
greater than three orders of magnitude, Gemini XS features
“Auto PMT Gain” to avoid saturating the photomultiplier tube.
The signal is calibrated against an internal standard, so the
reported RFU values of individual samples can be accurately
compared. |
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SoftMax Pro software
SoftMax Pro allows you to select
excitation and emission values and simply input their values for
the monochromator settings. You can easily scan both excitation
and emission spectra and determine the optimal settings.
PC/MAC Softmax Pro can be purchased new.
Engineered for superior performance and reliability
Gemini dual monochromator design —
an excitation monochromator (A) and emission monochromator (B) —
allows user-defined variable wavelength settings. Sending light
through a movable scanning grating (D) which selects the desired
excitation wavelength. The light is passed through 1 mm fiber
optic bundles (E) to elliptical focusing mirrors (F) that maximize
light collection efficiency for superior sensitivity and send the
beam to a microplate well (G). If fluorescent molecules are
present, the light of the emission wavelength is emitted back out
to focusing mirrors (H) that direct the light through 4 mm fiber
optic bundles (J) to the emission scanning grating (K). The
desired wavelength of light is then detected by the
photomultiplier tube, or PMT (L). The PMT passes the fluorescence
signal through the Gemini’s electronics, creating the signals that
result in data points collected by software. |
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Specifications** |
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Fluorescence photometric performance |
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Dual
Monochromators |
1 nm
increment selection |
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EX
Wavelength |
250-850
nm |
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EM
Wavelength |
360-850
nm |
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Bandwidth (Excitation & Emission) |
9 nm |
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Detection Limit (Signal 3X SD of Baseline) |
2.0 fmol/well
FITC 200 µL in 96 wells |
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Time Resolved Fluorescence (secondary mode) |
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Data
Collection |
50-1450 µsec, 200 µsec increments |
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Sensitivity |
0.5 fmol/well |
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Luminescence Photometric Performance |
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Wavelength
Range 250-850nm |
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Detection Limit |
10 amol/well
Alkaline Phosphatase 200 µl/well |
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General photometric specifications |
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Microplate Formats |
6-, 12-,
24-, 48-, 96-, and 384-well |
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Light
Source |
Xenon
flash lamp (1 joule/flash) |
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Detector |
Photomultiplier (R-3896) |
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Read
Time*** |
96 wells
in <27 seconds
384 wells in <45 seconds |
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Shaker
Time |
0 to 999
seconds |
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Temperature Control (Chamber) |
Ambient
+ 4 °C, to 45 °C |
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Catalog # 6723117 |
Softmax
Pro PC/MAC Software via interface connections
RS 232 serial
& parallel port |
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Catalog # 6723119 |
SpectraTest ABS 1
validation package |
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Catalog # P97040 |
Fully remanufactured with
one year warranty |
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Comes complete with line
cord, manual, six days a week technical support
and one year warranty. Softmax Pro PC/MAC Software additional. |
