Molecular Devices Gemini XS does both! Fluoro and Lumino

The Gemini XS spectrofluorometer allows for fast, high-sensitivity measurements. Cellular and molecular binding studies, DNA quantitation, drug screening, and SNP genotyping analysis all require a high degree of sensitivity. The Gemini system offers a flexible, filter-free environment allowing the determination of optimal excitation and emission settings in 1.0 nm increments providing the best level of sensitivity for variable assay conditions.

Instrument Description and Capabilities:

Fluorescence
Time Resolved Fluorescence TRF
Fluorescence Intensity top and bottom capabilities FI
Fluorescence filters included in 1nm range
Temperature control and microplate-shaking included
Adjustable bandwidth range
Luminescence glow reading mode
6-384-well plate capability

Applications

		Cellular and molecular binding studies
		Nucleic acid quantitation
		Cytoxicity assays
		SNP genotype detection and molecular beacons
		Enzyme activity
		CatchPoint^® cAMP Assay Kit

Features

		Dual Monochromators — The right pair of excitation and emission wavelengths is always available because the dual monochromators allow the selection of any wavelength in 1 nm increments. New fluorophores can easily be evaluated without purchasing additional filters.
		Wavelength Scanning — The most sensitive results are achieved by using optimal excitation and emission wavelengths. Literature wavelengths are often based on results from wavelength-limited, filter-based readers. Wavelength scanning ensures that the most sensitive assay conditions are used.
		Well Scanning — Gemini XS can report a single point from the well center, or multiple data points from large well tissue culture plates. This provides high sensitivity for cell-based assays.
		Auto PMT Gain — Because a single microplate often presents a range of fluorescence intensities greater than three orders of magnitude, Gemini XS features “Auto PMT Gain” to avoid saturating the photomultiplier tube. The signal is calibrated against an internal standard, so the reported RFU values of individual samples can be accurately compared.

SoftMax Pro software

SoftMax Pro allows you to select excitation and emission values and simply input their values for the monochromator settings. You can easily scan both excitation and emission spectra and determine the optimal settings. PC/MAC Softmax Pro can be purchased new.

Engineered for superior performance and reliability

Gemini dual monochromator design — an excitation monochromator (A) and emission monochromator (B) — allows user-defined variable wavelength settings. Sending light through a movable scanning grating (D) which selects the desired excitation wavelength. The light is passed through 1 mm fiber optic bundles (E) to elliptical focusing mirrors (F) that maximize light collection efficiency for superior sensitivity and send the beam to a microplate well (G). If fluorescent molecules are present, the light of the emission wavelength is emitted back out to focusing mirrors (H) that direct the light through 4 mm fiber optic bundles (J) to the emission scanning grating (K). The desired wavelength of light is then detected by the photomultiplier tube, or PMT (L). The PMT passes the fluorescence signal through the Gemini’s electronics, creating the signals that result in data points collected by software.

Specifications**

	Fluorescence photometric performance
	Dual Monochromators	1 nm increment selection
	EX Wavelength	250-850 nm
	EM Wavelength	360-850 nm
	Bandwidth (Excitation & Emission)	9 nm
	Detection Limit (Signal 3X SD of Baseline)	2.0 fmol/well FITC 200 µL in 96 wells
	Time Resolved Fluorescence (secondary mode)
	Data Collection	50-1450 µsec, 200 µsec increments
	Sensitivity	0.5 fmol/well
	Luminescence Photometric Performance
	Wavelength Range 250-850nm
	Detection Limit	10 amol/well Alkaline Phosphatase 200 µl/well
	General photometric specifications
	Microplate Formats	6-, 12-, 24-, 48-, 96-, and 384-well
	Light Source	Xenon flash lamp (1 joule/flash)
	Detector	Photomultiplier (R-3896)
	Read Time***	96 wells in <27 seconds 384 wells in <45 seconds
	Shaker Time	0 to 999 seconds
	Temperature Control (Chamber)	Ambient + 4 °C, to 45 °C
	Catalog # 6723117	Softmax Pro PC/MAC Software via interface connections RS 232 serial & parallel port
	Catalog # 6723119	SpectraTest ABS 1 validation package
	Catalog # P97040	Fully remanufactured with one year warranty (3 in stock)

Warranty information:

Instruments come complete with NIST certification and documentation, line cord and six days a week technical support. Remanufactured model with one year warranty. High quality instrument made in USA. Easily reset to 220 Volt.

Microplate Instrumentation Sales, Service and Support since 1982.

Applications

Features

SoftMax Pro software SoftMax Pro allows you to select excitation and emission values and simply input their values for the monochromator settings. You can easily scan both excitation and emission spectra and determine the optimal settings. PC/MAC Softmax Pro can be purchased new.

Engineered for superior performance and reliability

Specifications**

Fluorescence photometric performance

Time Resolved Fluorescence (secondary mode)

Luminescence Photometric Performance

General photometric specifications

SoftMax Pro software

SoftMax Pro allows you to select excitation and emission values and simply input their values for the monochromator settings. You can easily scan both excitation and emission spectra and determine the optimal settings. PC/MAC Softmax Pro can be purchased new.