| Multiskan Ascent offers superior
photometric measurement performance for 96- and 384-well plates. This
instrument reads the plate rapidly and can be connected to robotic
systems for increased throughput in photometric reading.
Excellent optical performance for 96- and
384-well plates
The high-quality optical system of the
Multiskan Ascent ensures excellent reproducibility, accuracy and
reliable results. The unit is linear up to an outstanding 4 Abs. units
with 96-well plates and up to 3 Abs. units with 384-well plates,
eliminating the need to dilute samples. This saves time in obtaining
sample results.
A recent validation study in a
high-throughput (HTS) environment with 384-well plates showed
excellent consistency of the signal across all wells in the plate
(low intraplate variation). No bias was observed in the IC50
as a result of curves being set up in different parts of the plate or
between two separate plates (interplate variation). A good
correlation was also observed in two different experiments using the
same group of compounds (low interassay variation).
Fast measurements
Multiskan Ascent provides not only
extreme accuracy and precision, but also very rapid measurements: It
takes only 9 seconds to measure a 96-well plate and only 20 seconds to
measure a 384-well plate. These values can have a noticeable effect on
a laboratory’s throughput.
Easy and flexible robot integration
Another feature that makes Multiskan
Ascent ideal for high-throughput environments is its simple and easy
robot integration. This unit is designed for integration, which can
increase walk-away reading capacity to 20 or 80 microplates.
Optional incubator
An optional internal incubator can be included for assays requiring
temperature control. Temperature can reach up to 50°C.
Specifications
Optical performance
Wavelength range |
340 nm -850 nm |
|
Filters |
8-position filter wheel with 405, 450, 490
and 630nm or filters of choice |
|
Half bandwidth of filters |
3-9 nm |
|
Wavelength accuracy |
+/- 2 nm |
|
Detector |
One silicon photo detector |
|
Linearity (96-well plate) |
0-4 Abs, +/- 2 %
0-3 Abs, +/- 2 %, 340 nm and 405 nm
0-3 Abs, +/- 2 % with continuous mode |
|
Linearity (384-well plate) |
0-3 Abs, +/- 2 %
0-2 Abs, +/- 2 % with continuous mode |
|
Read-out range |
0-6 Abs |
|
Resolution |
0.001 Abs |
|
Accuracy |
+/- 1 % or 0.003 Abs, whichever is greater (0-2 Abs)
+/- 2 % (2-3 Abs) |
|
Precision |
CV < 0.2 % (0-3 Abs),
CV < 1.0 % (3-4 Abs) stepping mode
CV < 0.3 % (0-2 Abs),
CV < 1.2 % (2-3 Abs) continuous mode |
|
Measurement time |
96-well plate 384-well plate |
|
Continuous mode |
9 s 20 s |
|
Stepping mode |
14 s 42 s |
Optional incubator
Temperature range
Catalog #2803540 |
Ambient + 4°C - 50°C
|
|
Uniformity |
+/- 0.8°C across the plate |
|
Shaking |
Linear shaking , 3 speeds |
|
Catalog # |
P97266 New |
|
Catalog # |
P97265
Demo |
|