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The Nepheloskan Ascent,
the first-ever microplate nephelometer (1990's), combines the sensitivity of a
nephelometer with the high throughput and economy of 96-well formats.
Together with Ascent Software, Nepheloskan Ascent offers unlimited
flexibility for research and for creating specific, easy-to-use procedures
for routine lab tests. The onboard incubator contains two heating element
plates in the measurement chamber, one heating element plate under the
microplate and another above it. Both heating element plates are
temperature-controlled. The upper plate is slightly warmer than the
lower plate to avoid condensation on the possible plate lid.
- First-ever microplate
nephelometer for particle measurements
- Advanced optical system
for unrivaled sensitivity
- Ascent Software for assay
optimization
Nepheloskan Ascent
application areas:
- Testing and development
of pharmaceuticals
- Measurements of
antimicrobial effects
- Bacterial growth (growth
curves)
- Development of growth
medium
- Quantitation of proteins
- Quality control of
processes
- Solubility testing
Application areas:
Nepheloskan Ascent allows
convenient testing of particles in 96 well microplate format for application such as
drug solubility tests, quantitation of specific proteins and antibiotic
sensitivity studies.
The incubator and adjustable
orbital shaker provide an added advantage for studies of factors affecting
the solubility of pharmaceuticals components as well as bacterial growth.
With the highly visual Ascent Software, the real-time kinetic curves of the
assay can be followed.
Several samples can be run simultaneously, saving time compared with
conventional methods.
In general, the light
scattering level of nephelometers and their sensitivity are studied by
formazine suspension or latex beads, which are commercially available.
Along with better sensitivity of Nepheloskan Ascent compared with
photometers, colors in a measurement medium do not interfere with the
quantitation of particles in a liquid form.
The measurement chamber is large to adopt
different plate formats and therefore extensive evaporation may cause some
variations in the temperatures between the wells. Consequently, when
using incubations with extended periods of time in the instrument, a plate
lid is recommended (lid has to be removed during measurement.)
Syringe volume 1000µl. Dead volume 600µl, Metal-free fluid
path.
Technical Specifications:
| Suggested Plate types: |
Sterile 96-well plate
use with or
without lids |
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Technical
Specifications:
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| Light source: |
Quartz halogen lamp in
stock |
| Detector: |
Photomultiplier tube |
| Spectral Response: |
580 to 630nm |
| Measurement Angle: |
Approprixmate 30° |
| Measurement Speed: |
Depends on the measurement
type, minimum 25 sec./plate. Acceleration and maximum speed are
adjustable. |
| Integration Time: |
20 to 1000 ms. Min. time 20
ms during which time the instrument sums 150 readings form the A/D
converter. |
| Shaker: |
Orbital method, speed
60-1200 rpm, diameter 1-50 mm |
| Temperature Range: |
Ambient temperature +3ºC to +45ºC |
| Recommended assay volume |
300µl per well |
| Injector
Syringe (option) |
1000µl.
Dead volume 600µl |
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| P21034 |
Nepheloskan Ascent Reader/Analyzer
(Several in stock) |
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P21035 |
Nepheloskan Ascent Reader/Analyzer
with 1 injector |
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