What the Instrument
The Discovery instrument is a high throughput
screening device for drug discovery that uses HTRF assay method
Homogeneous Time Resolved Fluorescence. It
handles microplate formats 96 and 384 wells, to provide flexibility and convenience for all
High throughput screening, basic screening and Assay development. This
instrument fires a nitrogen laser (337nm) at prepared samples and measures
the amount of fluorescence at two unique wavelengths: 620nm and 665nm.
The assay components are
labeled with two reagents: europium cryptate and XL665. Labeled assay
compontents are mixed with test compounds and are then ready to load into
Discovery. A laser beam is fired at each sample as the samples are read.
The light emission of both wavelengths are then measured.
The unbound cryptate reagent has a long-lived emission at 620nm.
The unbound XL665 reagent has a short lived emission at 665nm. When
the two reagents are in close proximity a non-radiative energy transfer
occurs which results in a long-lived emission at 665nm. The unique
energy spectra of the europium cryptate and XL665 labels eliminates the
need for separation and makes this technology homogeneous. The ratio
of the long-lived 665nm emission to the long-lived 620nm emission is a
measure of the bound assay components to the free assay components.
- Fluorescence (VIS range)
- Homogeneous Time Resolved Fluorescence
- Fluorescence Intensity top
and bottom capabilities FI
- Temperature control and microplate-shaking included
- 96 and 384 well plate capability
comes with a 90 day warranty and six days a week technical support. Easy operation with Packard
Hologram and Discovery PC software, accurate and precision
MTX Lab Systems, Inc.
Sales, service and support since 1982.