Measurement Principles

Fluorescence
Time-Resolved Fluorescence
Absorbance

Light Source

High-energy xenon flash lamp

Detector

Side window, current type photomultiplier tube

Filters

Two filter wheels for 8 filters each with your choice of 2 filter pairs

Gain Control

Automatic gain adjustment

Fluorescence Polarization

<5 mP standard deviation at 1 nm fluorescein

Fluorescence Intensity

Limit of detection: 0.9 fmol / well fluorescein
calculated according to the IUPAC standard: 3(SDblank)/slope

Time-Resolved Fluorescence

Limit of detection: 50 amol/well europium
calculated according to the IUPAC standard: 3(SDblank)/slope

Absorbance

Dynamic range: 0.000-3.000 OD
Reproducibility: 0.010 OD for 0-2 OD range

Microplate Formats

up to 384 and Fluorescence intensity and time-resolved fluorescence up to 1536

Reading Time

Depends on test definition
shortest reading time: 15 s for 96-well plate, 30 s for 384-well plate and 60 s for 1536-well plate

Incubation

Incubation range from ambient +5 °C to 45 °C (option: 60 °C)
Temperature stability 0.2 °C
Temperature gradient < 0.5 °C

Reagent Injectors

Up to 2 built-in syringe pumps with direct measurement access includes:
Variable injection speed
Individual volumes for each well
Four injection events per well
Reagent back flushing

Shaking

Orbital or linear shaking, user definable time and amplitude