BMG Polarstar Fluorescence, TRF and Absorbance Microplate Reader

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Additional in-stock Multi-Detection and
Fluorescence Plate Readers:
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BMG Polarstar Fluorescence, TRF and Absorbance Microplate Reader
Equipped with UV-VIS Fluorescence and Absorbance Reader
for top- and bottom- read assays
with your choice of filter pairs!

Wide range of applications

The Polarstar is a fully automated microplate based multi-detection reader which incorporates different measurement principles:

               • Fluorescence
               • Time-Resolved Fluorescence
               • Absorbance

It is designed for the widest possible range of non-radioactive labeled applications:

• Ca²⁺ Measurements (e.g. Ca²⁺ Flux with Fura-2 or Indo-1)
• Enzyme Activity
• Cell Toxicity, Proliferation and Viability
• ATP Quantification
• Immunoassays

By integrating a high intensity xenon flash lamp and liquid light guide technology, the Polarstar can cover the wavelength range from 250 to 740 nm. The Polarstar can read all plate formats from 6- up to 384-well plates in all 4 measurement modes and 1536-well plate reading is possible in both fluorescence and time-resolved mode. The versatile optical system allows instant switching from top to bottom reading.

Incubation

Temperature-regulated heating plates above and below the entire plate movement area provide uniform incubation up to 45°C.

Endpoint, slow and fast kinetic measurements

Kinetic applications can be defined, with interval times from 100 ms to several hours. In addition, there is the possibility to measure where it is important in a kinetic study.

Multi-color detection

The fast filter wheels and the ability to define up to 8 sets of filter, each with individual gain and measurement timing parameters, allow analysis of multiple fluorophores in a single well (FRET, dual-emission, DNA-detection).

Specifications

Polarstar - Technical Specifiations
Measurement Principles	Fluorescence Time-Resolved Fluorescence Absorbance
Light Source	High-energy xenon flash lamp
Detector	Side window, current type photomultiplier tube
Filters	Two filter wheels for 8 filters each with your choice of 2 filter pairs
Gain Control	Automatic gain adjustment
Fluorescence Polarization	<5 mP standard deviation at 1 nm fluorescein
Fluorescence Intensity	Limit of detection: 0.9 fmol / well fluorescein calculated according to the IUPAC standard: 3(SDblank)/slope
Time-Resolved Fluorescence	Limit of detection: 50 amol/well europium calculated according to the IUPAC standard: 3(SDblank)/slope
Absorbance	Dynamic range: 0.000-3.000 OD Reproducibility: 0.010 OD for 0-2 OD range
Microplate Formats	up to 384 and Fluorescence intensity and time-resolved fluorescence up to 1536
Reading Time	Depends on test definition shortest reading time: 15 s for 96-well plate, 30 s for 384-well plate and 60 s for 1536-well plate
Incubation	Incubation range from ambient +5 °C to 45 °C (option: 60 °C) Temperature stability 0.2 °C Temperature gradient < 0.5 °C
Reagent Injectors	Up to 2 built-in syringe pumps with direct measurement access includes: Variable injection speed Individual volumes for each well Four injection events per well Reagent back flushing
Shaking	Orbital or linear shaking, user definable time and amplitude
Stacker	For automatic plate handling and on some models, a Stacker is available with magazines for up to 25 plates (6-1536 well plate formats) and barcode reader with flexible barcode handling. Please check with manufacturer on your model
Catalog #P21006 Polarstar Remanufactured with one year warranty.

Incubation Temperature-regulated heating plates above and below the entire plate movement area provide uniform incubation up to 45°C.

Incubation

Temperature-regulated heating plates above and below the entire plate movement area provide uniform incubation up to 45°C.