BMG Lab Technologies Galaxy Reader

Microplate Instrumentation Specialists

          Ordering Information
          Phone 1.800.848.6474
          Phone 01.703.821.1045
          Fax      01.703.821.1046
          Email   info@mtxlsi.com

Four measurement principles for the widest range of applications

The Polarstar is a fully automated microplate based multi-detection reader which incorporates four different measurement principles:

               • Fluorescence
               • Fluorescence Intensity
               • Time-Resolved Fluorescence
               • Absorbance

It is designed for the widest possible range of non-radioactive labeled applications:

• Ca²⁺ Measurements (e.g. Ca²⁺ Flux with Fura-2 or Indo-1) • Enzyme Activity • Cell Toxicity, Proliferation and Viability • ATP Quantification • Immunoassays
By integrating a high intensity xenon flash lamp and liquid light guide technology, the Galaxy can cover the wavelength range from 250 to 740 nm. The Galaxy can read plate formats of 96-well plates in all 4 measurement modes. Top to bottom readings.

Incubation

Temperature-regulated heating plates above and below the entire plate movement area provide uniform incubation up to 45°C.

Endpoint, slow and fast kinetic measurements

Kinetic applications can be defined, with interval times from 100 ms to several hours. In addition, there is the possibility to measure where it is important in a kinetic study.

Multi-color detection

The fast filter wheels and the ability to define up to 8 sets of filter, each with individual gain and measurement timing parameters, allow analysis of multiple fluorophores in a single well (FRET, dual-emission, DNA-detection).

Specifications

Polarstar - Technical Specifiations
Measurement Principles	Fluorescence Fluorescence Intensity Time-Resolved Fluorescence Absorbance
Light Source	High-energy xenon flash lamp
Detector	Side window, current type photomultiplier tube
Filters	Two filter wheels for 8 filters each with your choice of 2 filter pairs of choice
Gain Control	Automatic gain adjustment
Fluorescence Intensity	Limit of detection: 0.9 fmol / well fluorescein calculated according to the IUPAC standard: 3(SDblank)/slope
Time-Resolved Fluorescence	Limit of detection: 50 amol/well europium calculated according to the IUPAC standard: 3(SDblank)/slope
Absorbance	Dynamic range: 0.000-3.000 OD Reproducibility: 0.010 OD for 0-2 OD range
Microplate Formats	up to 96 well Fluorescence intensity and time-resolved fluorescence up to 1536 well
Reading Time	Depends on test definition shortest reading time: 15 s for 96-well plate
Incubation	Incubation range from ambient +5 °C to 45 °C (option: 60 °C) Temperature stability 0.2 °C Temperature gradient < 0.5 °C
Reagent Injectors Option	Up to 2 built-in syringe pumps with direct measurement access includes: Variable injection speed Individual volumes for each well Four injection events per well Reagent back flushing Check with manufacturer on your model*
Shaking	Orbital or linear shaking, user definable time and amplitude
Dimensions	LxWxH: 48 x 44 x 27 cm; weight 28 kg
Catalog #P21008 Polarstar Galaxy Remanufactured with one year warranty.

Incubation Temperature-regulated heating plates above and below the entire plate movement area provide uniform incubation up to 45°C.

Incubation

Temperature-regulated heating plates above and below the entire plate movement area provide uniform incubation up to 45°C.