Molecular Devices SpectraMax M2 Microplate Reader

Molecular Devices SpectraMax M2 Microplate Reader SOLD
Multi-Detection Reader and Dual-Mode Cuvette Port

SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint, kinetic, spectrum and area-well scanning read types and PathCheck® allow homogeneous and heterogeneous microplate assays to be performed in this flexible system. The FI and Abs microplate reader with built-in cuvette port allows easy conversion and optimization of very-low-throughput assays into medium-high-throughput microplate assays. The SpectraMax M2 allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command.

Applications

		DNA/RNA/protein quantitation and purity
		PicoGreen®/NanoOrange™/Bradford
		ELISAs/enzyme kinetics (i.e., K_m, K_i, etc.)
		Drug dissolution profiles
		Live/Dead® Viability/cytotoxicity assays
		Solubility assays
		Permeability assays (PAMPA)
		Human Serum Albumin binding assays
		Lipophilicity assays
		ADME-Tox assays
		CatchPoint® cAMP assays
		Caspase-3 and protease assays

Features

		Multi-detection ability
		Dual-mode cuvette port
		Dual monochromators
		Instrument & software validation
		Robotics compatible
		Pathcheck® and well volume sensors

Compound Profiling Assays

SpectraMax microplate readers are ideal for increased throughput of early ADME compound profiling assays such as the Transil® Membrane Affinity assay and Human Serum Albumin Binding assay. The Membrane Affinity assay provides high-throughput lipophilicity data equivalent to liposome-based methods without the need for ultracentrifugation. The Human Serum Albumin Binding assay provides high-throughput blood protein binding data by measuring the fraction bound to Human Serum Albumin.

Specifications

absorbance photometric performance
Wavelength Range	200-1000 nm
Wavelength Selection	Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth	< 4.0 nm
Wavelength Accuracy	±2.0 nm
Wavelength Repeatability	±0.2 nm
Photometric Range	0-4.0 OD
Photometric Resolution	0.001 OD
Photometric Accuracy (Microplate)	< ±0.006 OD ±1.0%, 0-2 OD
Photometric Accuracy (Cuvette)	< ±0.005 OD ±1.0%, 0-2 OD
Photometric Precision	< ±0.003 OD ±1.0%, 0-2 OD
Baseline Flatness	< 0.001 OD
Stray Light	< 0.05% @ 230 nm
fluorescence photometric performance
Dual Monochromators	1 nm increments EX 250-850 nm EM 360-850 nm
Bandwidth (EX, EM)	9, 9 nm
Detection Limit	3.0 fmol/well FITC 200 µlin 96 wells (signal 3X std. dev. of baseline)
time-resolved fluorescence (secondary mode)
Wavelength Range	360-850 nm
Data Collection	50-1450 µsec, 200 µsec increments
Sensitivity	0.5 fmol/well Eu-chelate (obtained with DELFIA® reagent from Perkin Elmer by using a 384-well plate)
luminescence (secondary mode)
Wavelength Range	360-850 nm
Detection Limit	10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II™ reagent from Applied Biosystems)
general performance
Plate Formats	6, 12, 24, 48, 96, 384 wells
Light Source	Xenon flash lamp (1 joule/flash)
Detector	Photomultiplier (R-3896)
Read Time** 96-well 384-well	Abs 18 sec., FI 15 sec. Abs 49 sec., FI 45 sec.
Shaker Time	0 to 999 seconds
Temperature Range	Ambient +4 °C to 45 °C
Temperature Uniformity	±0.5 °C @ 37 °C set pt.
Temperature Accuracy	±1 °C @ 37 °C

Comes with PC Software, line cord, six days a week technical support and one year warranty.

Applications

Features

Compound Profiling Assays

absorbance photometric performance

fluorescence photometric performance

time-resolved fluorescence (secondary mode)

luminescence (secondary mode)

general performance