Contact Us: 1.703.821.1045 info@mtxlsi.com
Fax: 1.703.821.1046


Additional in-stock Multi-mode Detection
and Fluorescence Plate Readers:
Click on link to go to page


|Fluoroskan I|Perseptive Biosystems Cytofluor 4000|BMG FLUOstar|

|Biotek FL 600|Dynex Technologies MLX|

|ThermoFisher Finstruments Fluorescence|ThermoFisher Ascent|

|Tecan Spectrafluor|Tecan Spectrafluor Plus|Tecan Genios|

|Molecular Devices Gemini XS|ThermoFisher Luminoskan|

|ThermoFisher Luminoskan Ascent|

|BMG FLUOstar Galaxy|Perkin Elmer Victor 2 with or w/o injectors|

|BMG POLARstar|

|BMG POLARstar Galaxy|Biotek FLX 800|Tecan Ultra|

|Molecular Devices Gemini EM|Tecan Safire|

Molecular Devices SpectraMax M2 Microplate Reader SOLD 
Multi-Detection Reader and Dual-Mode Cuvette Port



 

SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint, kinetic, spectrum and area-well scanning read types and PathCheck® allow homogeneous and heterogeneous microplate assays to be performed in this flexible system. The FI and Abs microplate reader with built-in cuvette port allows easy conversion and optimization of very-low-throughput assays into medium-high-throughput microplate assays. The SpectraMax M2 allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command.
 
 

Applications

    DNA/RNA/protein quantitation and purity
    PicoGreen®/NanoOrange™/Bradford
    ELISAs/enzyme kinetics (i.e., Km, Ki, etc.)
    Drug dissolution profiles
    Live/Dead® Viability/cytotoxicity assays
Solubility assays
Permeability assays (PAMPA)
    Human Serum Albumin binding assays
    Lipophilicity assays
    ADME-Tox assays
    CatchPoint® cAMP assays
    Caspase-3 and protease assays

Features

    Multi-detection ability
    Dual-mode cuvette port
    Dual monochromators
    Instrument & software validation
    Robotics compatible
    Pathcheck® and well volume sensors
 

 
 

Compound Profiling Assays

SpectraMax microplate readers are ideal for increased throughput of early ADME compound profiling assays such as the Transil® Membrane Affinity assay and Human Serum Albumin Binding assay. The Membrane Affinity assay provides high-throughput lipophilicity data equivalent to liposome-based methods without the need for ultracentrifugation. The Human Serum Albumin Binding assay provides high-throughput blood protein binding data by measuring the fraction bound to Human Serum Albumin.

 

 
  Specifications

absorbance photometric performance

Wavelength Range 200-1000 nm
Wavelength Selection Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth < 4.0 nm
Wavelength Accuracy ±2.0 nm
Wavelength Repeatability ±0.2 nm
Photometric Range 0-4.0 OD
Photometric Resolution 0.001 OD
Photometric Accuracy (Microplate) < ±0.006 OD ±1.0%, 0-2 OD
Photometric Accuracy (Cuvette) < ±0.005 OD ±1.0%, 0-2 OD
Photometric Precision < ±0.003 OD ±1.0%, 0-2 OD
Baseline Flatness < 0.001 OD
Stray Light < 0.05% @ 230 nm

fluorescence photometric performance

Dual Monochromators 1 nm increments
EX 250-850 nm
EM 360-850 nm
Bandwidth (EX, EM) 9, 9 nm
Detection Limit 3.0 fmol/well FITC 200 µlin 96 wells (signal 3X std. dev. of baseline)

time-resolved fluorescence (secondary mode)

Wavelength Range 360-850 nm
Data Collection 50-1450 µsec, 200 µsec increments
Sensitivity 0.5 fmol/well Eu-chelate (obtained with DELFIA® reagent from Perkin Elmer by using a 384-well plate)

luminescence (secondary mode)

Wavelength Range 360-850 nm
Detection Limit 10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II™ reagent from Applied Biosystems)

general performance

Plate Formats 6, 12, 24, 48, 96, 384 wells
Light Source Xenon flash lamp (1 joule/flash)
Detector Photomultiplier (R-3896)
Read Time** 96-well
384-well
Abs 18 sec., FI 15 sec.
Abs 49 sec., FI 45 sec.
Shaker Time 0 to 999 seconds
Temperature Range Ambient +4 °C to 45 °C
Temperature Uniformity ±0.5 °C @ 37 °C set pt.
Temperature Accuracy ±1 °C @ 37 °C
   

Comes with  PC Software, line cord,  six days a week technical support and one year warranty. 

 
Copyright © 2010. All Rights Reserved HOME | FLUORESCENCE | WASHERS | READERS | PIPETTORS | DISPENSERS | CONTACT