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and Fluorescence Plate Readers:
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|Fluoroskan I|Perseptive Biosystems Cytofluor 4000|BMG FLUOstar|

|Biotek FL 600|Dynex Technologies MLX|

|ThermoFisher Finstruments Fluorescence|ThermoFisher Ascent|

|Tecan Spectrafluor|Tecan Spectrafluor Plus|Tecan Genios|

|Molecular Devices Gemini XS|ThermoFisher Luminoskan|

|ThermoFisher Luminoskan Ascent|

|BMG FLUOstar Galaxy|Perkin Elmer Victor 2 with or w/o injectors|

|BMG POLARstar|

|BMG POLARstar Galaxy|Biotek FLX 800|Tecan Ultra|

|Molecular Devices Gemini EM|Tecan Safire|


Molecular Devices Spectramax M5 w/cuvette port
Absorbance, Fluorescence, FI, TRF, Polarization and Luminescence Reader
Multi-Detection Reader and Dual-Mode Cuvette Port



 

Spectramax M5 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Detection include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint, kinetic, spectrum and area-well scanning read types and PathCheck® allow homogeneous and heterogeneous microplate assays to be performed in this flexible system. The FI and Abs microplate reader with built-in cuvette port allows easy conversion and optimization of very-low-throughput assays into medium-high-throughput microplate assays. The SpectraMax M5e allows faster, more precise results and reagent savings and is the top of the line instrument. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command.
 
 

Applications

    DNA/RNA/protein quantitation and purity
    PicoGreen®/NanoOrange™/Bradford
    ELISAs/enzyme kinetics (i.e., Km, Ki, etc.)
    Drug dissolution profiles
    Live/Dead® Viability/cytotoxicity assays
Solubility assays
Permeability assays (PAMPA)
    Human Serum Albumin binding assays
    Lipophilicity assays
    ADME-Tox assays
    CatchPoint® cAMP assays
    Caspase-3 and protease assays

Features

    Multi-detection ability
    Dual-mode cuvette port
    Dual monochromators
    Instrument & software validation
    Robotics compatible
    Pathcheck® and well volume sensors
 

 
 

Compound Profiling Assays

Spectramax microplate readers are ideal for increased throughput of early ADME compound profiling assays such as the Transil® Membrane Affinity assay and Human Serum Albumin Binding assay. The Membrane Affinity assay provides high-throughput lipophilicity data equivalent to liposome-based methods without the need for ultracentrifugation. The Human Serum Albumin Binding assay provides high-throughput blood protein binding data by measuring the fraction bound to Human Serum Albumin.

 

 
  Specifications

Absorbance photometric performance

Wavelength Range 200-1000 nm
Wavelength Selection Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth < 4.0 nm
Wavelength Accuracy ±2.0 nm
Wavelength Repeatability ±0.2 nm
Photometric Range 0-4.0 OD
Photometric Resolution 0.001 OD
Photometric Accuracy (Microplate) < ±0.006 OD ±1.0%, 0-2 OD
Photometric Accuracy (Cuvette) < ±0.005 OD ±1.0%, 0-2 OD
Photometric Precision < ±0.003 OD ±1.0%, 0-2 OD
Baseline Flatness < 0.001 OD
Stray Light < 0.05% @ 230 nm

Fluorescence photometric performance

Dual Monochromators 1 nm increments
EX 250-850 nm
EM 360-850 nm
Bandwidth (EX, EM) 9, 9 nm
Detection Limit 3.0 fmol/well FITC 200 µlin 96 wells (signal 3X std. dev. of baseline)

Time-resolved fluorescence (secondary mode)

Wavelength Range 360-850 nm
Data Collection 50-1450 µsec, 200 µsec increments
Sensitivity 0.5 fmol/well Eu-chelate (obtained with DELFIA® reagent from Perkin Elmer by using a 384-well plate)

Luminescence (secondary mode)

Wavelength Range 360-850 nm
Detection Limit 10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II™ reagent from Applied Biosystems)

General performance

Plate Formats 6, 12, 24, 48, 96, 384 wells
Light Source Xenon flash lamp (1 joule/flash)
Detector Photomultiplier (R-3896)
Read Time** 96-well
384-well
Abs 18 sec., FI 15 sec.
Abs 49 sec., FI 45 sec.
Shaker Time 0 to 999 seconds
Temperature Range Ambient +4 °C to 45 °C
Temperature Uniformity ±0.5 °C @ 37 °C set pt.
Temperature Accuracy ±1 °C @ 37 °C
Catalog #P97048 Spectramax M5
   

Comes with line cord, six days a week technical support
 and one year warranty. 

 
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