Molecular Devices Spectramax M5 w/cuvette port Absorbance, Fluorescence, FI, TRF, Polarization and Luminescence Reader

Spectramax M5 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Detection include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint, kinetic, spectrum and area-well scanning read types and PathCheck® allow homogeneous and heterogeneous microplate assays to be performed in this flexible system. The FI and Abs microplate reader with built-in cuvette port allows easy conversion and optimization of very-low-throughput assays into medium-high-throughput microplate assays. The SpectraMax M5e allows faster, more precise results and reagent savings and is the top of the line instrument. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command.

Applications

		DNA/RNA/protein quantitation and purity
		PicoGreen®/NanoOrange™/Bradford
		ELISAs/enzyme kinetics (i.e., K_m, K_i, etc.)
		Drug dissolution profiles
		Live/Dead® Viability/cytotoxicity assays
		Solubility assays
		Permeability assays (PAMPA)
		Human Serum Albumin binding assays
		Lipophilicity assays
		ADME-Tox assays
		CatchPoint® cAMP assays
		Caspase-3 and protease assays

Features

		Multi-detection ability
		Dual-mode cuvette port
		Dual monochromators
		Instrument & software validation
		Robotics compatible
		Pathcheck® and well volume sensors

Compound Profiling Assays

Spectramax microplate readers are ideal for increased throughput of early ADME compound profiling assays such as the Transil® Membrane Affinity assay and Human Serum Albumin Binding assay. The Membrane Affinity assay provides high-throughput lipophilicity data equivalent to liposome-based methods without the need for ultracentrifugation. The Human Serum Albumin Binding assay provides high-throughput blood protein binding data by measuring the fraction bound to Human Serum Albumin.

Specifications

Absorbance photometric performance
Wavelength Range	200-1000 nm
Wavelength Selection	Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth	< 4.0 nm
Wavelength Accuracy	±2.0 nm
Wavelength Repeatability	±0.2 nm
Photometric Range	0-4.0 OD
Photometric Resolution	0.001 OD
Photometric Accuracy (Microplate)	< ±0.006 OD ±1.0%, 0-2 OD
Photometric Accuracy (Cuvette)	< ±0.005 OD ±1.0%, 0-2 OD
Photometric Precision	< ±0.003 OD ±1.0%, 0-2 OD
Baseline Flatness	< 0.001 OD
Stray Light	< 0.05% @ 230 nm
Fluorescence photometric performance
Dual Monochromators	1 nm increments EX 250-850 nm EM 360-850 nm
Bandwidth (EX, EM)	9, 9 nm
Detection Limit	3.0 fmol/well FITC 200 µlin 96 wells (signal 3X std. dev. of baseline)
Time-resolved fluorescence (secondary mode)
Wavelength Range	360-850 nm
Data Collection	50-1450 µsec, 200 µsec increments
Sensitivity	0.5 fmol/well Eu-chelate (obtained with DELFIA® reagent from Perkin Elmer by using a 384-well plate)
Luminescence (secondary mode)
Wavelength Range	360-850 nm
Detection Limit	10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II™ reagent from Applied Biosystems)
General performance
Plate Formats	6, 12, 24, 48, 96, 384 wells
Light Source	Xenon flash lamp (1 joule/flash)
Detector	Photomultiplier (R-3896)
Read Time** 96-well 384-well	Abs 18 sec., FI 15 sec. Abs 49 sec., FI 45 sec.
Shaker Time	0 to 999 seconds
Temperature Range	Ambient +4 °C to 45 °C
Temperature Uniformity	±0.5 °C @ 37 °C set pt.
Temperature Accuracy	±1 °C @ 37 °C
Catalog #P97048	Spectramax M5

Applications

Features

Compound Profiling Assays

Absorbance photometric performance

Fluorescence photometric performance

Time-resolved fluorescence (secondary mode)

Luminescence (secondary mode)

General performance