| Instrument Description
and Capabilities: The SPECTRAFLUOR is a fully automatic, computer controlled
Fluorescence and Absorbance instrument for measuring samples in a microplate. The instrument
fulfills the requirements for numerous fluorescence and absorbance
applications in research and routine investigations.
- Fluorescence (VIS range)
- Time Resolved Fluorescence
- Fluorescence Intensity top
and bottom capabilities
- Fluorescence Resonanace Energy
Transfer FRET
- User choice of Fluorescence
filters included
- Absorbance reading mode (UV and VIS
range)
- User choice of Absorbance
filter included
- Temperature control and microplate-shaking included
- Adjustable bandwidth range
- 6-384-well plate capability
The filters are arranged in filter slides; four
excitation filters and four emission filters respectively as noted
below. Handles 6, 24, 96, 384
and 1536
well plates.
Spectral range 360-700nm for fluorescence.
Spectral range
230-750nm for absorbance.
Sensitivity ≤3pg fluorescein/
well or ≤8 fmol well. The emission signal is measured by a highly sensitive
PMT.
Different detection techniques with one instrument change of
measurement directions (top to bottom, bottom to top) by PC software. Thermal
incubation at +5º C to + 40º C.
Dual optical channels allow measurements from above or below the plate
by mouse-click. Light to and from the samples is focused by a lens and
high quality excitation and emission filters are easily accessible
from the front of the instrument. Each filter slide holds up to four
filters with eight filters onboard for fluorescence.
Orbital shaking and temperature control
are standard.
Suggested
use with black clear bottomed plates for bottom-to-top readings.
Optimal use with black plates prevent cross-contamination. |
Specifications:
| Modes of Operation |
|
| Light source |
High energy Xenon flash lamp |
| Detector |
Side window, low dark current
photomultiplier tube |
| Plate Shaking 3 intensities |
Linear and Orbital shaking with three different speeds |
| Fluorescence Spectral Filter Wavelength Range |
340 - 700 nm |
| Fluorescence Sensitivity 8fmol (FEMTO
MOLE) |
≤3pg fluorescein 200μL
≤8fmol/well in black plate |
Excitation Filters
(Choice of 2 filter pairs for Fluorescence readings) |
340 - 700 nm
Advise user's choice of filters |
Emission Filters
(Choice of 2 filter pairs for Fluorescence readings) |
340 - 700 nm
Advise user's choice of filters |
| Absorbance Spectral Filter Wavelength Range |
230-750nm |
| Absorbance Filters |
Advise user's choice
can be purchased |
| Absorbance Measurement and Precision at
492nm |
0-3 Optical Density |
| Temperature range |
+5º C to + 40º C |
| Microwell Plate Type |
6, 24, 96, 384
well plates
Fluorescence and Absorbance |
| Fluorescence Plate Types |
Black plates optimal
to prevent cross contamination |
| Interface connections |
RS 232 serial
& parallel port |
Ordering information |
Catalog #P97083 Remanufactured |
Dimensions: LxWxH: 48 x 44 x 27 cm; weight 28 kg
Warranty
information:
Instruments come complete with NIST
certification and documentation on absorbance filters, line cord and six days a week technical support. Remanufactured model
with warranty. These models sell new for $21,000.
High quality instrument manufactured in Austria and
Germany and sold worldwide. Easily reset to 220 Volt.
Microplate Instrumentation
Sales, Service and Support since 1982.
Fluorescence Filters and Applications click here!
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